{
  "abstract": "Prokaryotic antiviral type III CRISPR-Cas and CBASS systems use cyclic nucleotides as second messengers to activate diverse effector proteins. Whereas CBASS signaling typically triggers abortive infection, type III CRISPR-Cas signaling is tightly regulated through controlled signal production and degradation. Here, we define the molecular mechanism of the CRISPR-associated Csx15 protein family and demonstrate that Csx15 functions as a broad specificity ring nuclease capable of degrading cyclic nucleotide signals from both CRISPR-Cas and CBASS pathways. Structural and biochemical analyses reveal that Csx15 adopts diverse oligomeric states and undergoes ligand-induced conformational transitions that promote filament assembly required for catalytic activity, analogous to filament-forming SAVED-domain effectors. Finally, we provide evidence that Csx15 is a close relative of SAVED domains suggesting that Csx15-like proteins may have also contributed to the evolution of CBASS cyclic nucleotide signaling.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Biotechnology, Life Sciences Center, Vilnius University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "D. Klimavicius"
    },
    {
      "name": "D. Rafalski"
    },
    {
      "name": "D. Smalakyte"
    },
    {
      "name": "A. Ruksenaite"
    },
    {
      "name": "G. Sasnauskas"
    },
    {
      "name": "G. Tamulaitiene"
    },
    {
      "name": "C. Venclovas"
    },
    {
      "name": "M. Bochtler"
    },
    {
      "affs": [
        0
      ],
      "name": "G. Tamulaitis"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/czkbb1czb10k9.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.26.761840",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761840v1.full",
  "id": "czkbb1czb10k9",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761840v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7224119879",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761840v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/czkbb1czb10k9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:59Z",
      "native_id": "10.64898/2026.10.26.761840",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761840v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|CRISPR and Genetic Engineering",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Csx15 is a broad specificity ring nuclease bridging CRISPR-Cas and CBASS cyclic nucleotide signaling systems",
  "title_text": "Csx15 is a broad specificity ring nuclease bridging CRISPR-Cas and CBASS cyclic nucleotide signaling systems",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:59Z",
  "url": "https://research.draconitic.com/paper/czkbb1czb10k9",
  "venue": "bioRxiv",
  "versions": []
}