{
  "abstract": "Tumors contain a mixture of T cells with bystander reactivities and reactivities towards different, frequently patient-specific, cancer (neo)antigens. The one-step identification of highly active TCR-antigen pairs in human tumors would be valuable, both as a monitoring tool and to boost T cell reactivities of interest. Here, we develop PAIR-Scan, an HLA-agnostic library-on-library screening technology that identifies functionally active TCR-neoantigen pairs among tens of thousands of candidate pairs in a single step. We demonstrate the value of PAIR-Scan on a range of tumor samples and for the direct identification of TCR-recognized minimal peptides. In addition, we demonstrate that PAIR-Scan correctly ranks TCRs reactive to the same antigen by their relative tumor-killing efficiency. Together, these data demonstrate the value of PAIR-Scan for both the dissection of T cell responses in clinical samples and to generate large-scale datasets for the development of predictive models of TCR reactivity.",
  "abstract_shown": true,
  "affiliations": [
    "The Netherlands Cancer Institute"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 25,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "M. Messemaker"
    },
    {
      "name": "C. A. Richterich"
    },
    {
      "name": "J. Wenner"
    },
    {
      "name": "Y. Ma"
    },
    {
      "name": "Z. Moravec"
    },
    {
      "name": "R. Voogd"
    },
    {
      "name": "M. Mussmann"
    },
    {
      "name": "S. de Paauw"
    },
    {
      "name": "B. P. Y. Kwee"
    },
    {
      "name": "J. Geerligs"
    },
    {
      "name": "B. Morris"
    },
    {
      "name": "Y. Winkler"
    },
    {
      "name": "B. O. Springer"
    },
    {
      "name": "F. T. B. T. van den Brekel"
    },
    {
      "name": "J. Urbanus"
    },
    {
      "name": "A. Guislain"
    },
    {
      "name": "N. Alfaiate"
    },
    {
      "name": "F. van Diepen"
    },
    {
      "name": "M. van Baalen"
    },
    {
      "name": "B. Nicolet"
    },
    {
      "name": "J. B. A. G. Haanen"
    },
    {
      "name": "C. J. Wu"
    },
    {
      "name": "J. Huuhtanen"
    },
    {
      "name": "W. Scheper"
    },
    {
      "name": "T. N. Schumacher"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/czp53bw947x5f.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.07.749948",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.07.749948v1.full",
  "id": "czp53bw947x5f",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.07.749948v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "immunology"
    ],
    [
      "openalex",
      "2403|Immunotherapy and Immune Responses"
    ]
  ],
  "openalex": "W7213067321",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.07.749948v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/czp53bw947x5f.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:13Z",
      "native_id": "10.64898/2026.09.07.749948",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.07.749948v3"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "PAIR-Scan: Single-step identification of highly potent TCR-neoantigen pairs through library-on-library screening",
  "title_text": "PAIR-Scan: Single-step identification of highly potent TCR-neoantigen pairs through library-on-library screening",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:35Z",
  "url": "https://research.draconitic.com/paper/czp53bw947x5f",
  "venue": "bioRxiv",
  "versions": []
}