{
  "abstract": "Although advances in mass spectrometry now support proteomic screens of thousands of samples and sample preparation has been automated to match, most current protocols still start from pre-prepared cell lysates. Here, we present a protocol for automated mass spectrometry sample preparation starting at the cell culture plate level. We describe steps for cell seeding in 96-well plates, in-plate cell lysis, and single-pot solid-phase-enhanced sample preparation. This streamlined approach significantly reduces manual handling while yielding highly reproducible measurements from low cell numbers.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biology, University of Fribourg, Chemin Du Musée 10, 1700 Fribourg, Switzerland"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sibilla Sander"
    },
    {
      "affs": [
        0
      ],
      "name": "Sophie Buchser"
    },
    {
      "affs": [
        0
      ],
      "name": "Michael Stumpe"
    },
    {
      "affs": [
        0
      ],
      "name": "Joern Dengjel"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d05fas5vwbj1v.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104917",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735248/",
  "id": "d05fas5vwbj1v",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104917",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7224094035",
  "pdf_url": null,
  "pmcid": "PMC13735248",
  "pmid": "42949911",
  "published": "2026-10-28",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/d05fas5vwbj1v.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:20:57Z",
      "native_id": "PMC13735248",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104917"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Automated plate-to-peptide protocol for mass spectrometry sample preparation",
  "title_text": "Automated plate-to-peptide protocol for mass spectrometry sample preparation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:20:57Z",
  "url": "https://research.draconitic.com/paper/d05fas5vwbj1v",
  "venue": "STAR Protocols",
  "versions": []
}