{
  "abstract": "Glioblastoma research requires human models that preserve tumor heterogeneity and microenvironmental interactions. Here, we present a protocol for generating the human organoid tumor transplantation (HOTT) model and its immunocompetent counterpart, the immune-HOTT (iHOTT) model. These platforms use direct-from-patient tumor cells engrafted into organoids to recapitulate the tumor microenvironment and, in the iHOTT system, autologous tumor-immune interactions. We describe steps for organoid generation, patient tumor dissociation, co-culture with autologous peripheral blood mononuclear cells, fluorescence-activated cell sorting, and downstream single-cell RNA sequencing.\n\nFor complete details on the use and execution of this protocol, please refer to Ge et al. and Baisiwala et al.1,2",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA",
    "Department of Neurosurgery, UCLA Health, Los Angeles, CA 90095, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Matthew X. Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Weihong Ge"
    },
    {
      "affs": [
        0
      ],
      "name": "Elisa Fazzari"
    },
    {
      "affs": [
        0
      ],
      "name": "Aparna Bhaduri"
    },
    {
      "affs": [
        1
      ],
      "name": "Shivani Baisiwala"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d0zgb6hnt9k7x.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104925",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13733458/",
  "id": "d0zgb6hnt9k7x",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104925",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "2730|Glioma Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7224332062",
  "pdf_url": null,
  "pmcid": "PMC13733458",
  "pmid": "42961905",
  "published": "2026-10-30",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/d0zgb6hnt9k7x.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:23:14Z",
      "native_id": "PMC13733458",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104925"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Glioma Diagnosis and Treatment",
      "subfield": "oncology"
    }
  ],
  "title": "Protocol for generating human organoid tumor transplantation and immune-human organoid tumor transplantation models for glioblastoma research",
  "title_text": "Protocol for generating human organoid tumor transplantation and immune-human organoid tumor transplantation models for glioblastoma research",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:23:14Z",
  "url": "https://research.draconitic.com/paper/d0zgb6hnt9k7x",
  "venue": "STAR Protocols",
  "versions": []
}