{
  "abstract": "Human exposure to arsenic has been reported to induce metabolic disorders in pancreatic β-cells. However, metabolic fluxes of essential carbon sources in pancreatic β-cells under arsenic exposure were not quantitatively characterized. In this study, ¹³C-labeled glucose and glutamine were used as the tracers to examine their fates in INS-1 832/13 cells treated with arsenite (iAsIII). INS-1 832/13 cells exposed to three concentrations of iAsIII (0.05, 0.5, and 1.0 μg/mL) maintained at least 80.0% of cell viability in comparison to the controls. Glucose uptake of INS-1 832/13 cells exposed to 1.0 μg/mL iAsIII was approximately 2 times higher than that of the controls. Accordingly, glucose was also accumulated in the iAsIII-treated INS-1 832/13 cells. The activities of glucose-derived pathways in INS-1 832/13 cells, including glycolysis, tricarboxylic acid (TCA) cycle, and pentose phosphate pathway (PPP), were consistently enhanced by iAsIII. However, mitochondrial respiratory indexes of INS-1 832/13 cells, including oxygen consumption rate (OCR), ATP-linked OCR, basal OCR, maximal respiration, and spare respiratory capacity, were considerably suppressed by iAsIII. Inhibitive effects of iAsIII on metabolic pathway activities of glutamine were observed. Collectively, our results suggest that iAsIII exposure can induce metabolic reprogramming involved with glucose and glutamine in INS-1 832/13 cells.",
  "abstract_shown": true,
  "affiliations": [
    "School of Environmental Science and Engineering, School of Life and Health Sciences, Hainan University, Haikou 570228, China.",
    "State Key Laboratory of Biocontrol, School of Life Sciences, School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China.",
    "Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.",
    "Department of Endocrinology and Metabolism, Guangdong Provincial Key Laboratory of Diabetology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.",
    "Jiangmen Key Laboratory of Synthetic Chemistry and Cleaner Production, School of Environmental and Chemical Engineering, Wuyi University, Jiangmen 529020, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ruijia Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Liangyu Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Yingshi Lin"
    },
    {
      "affs": [
        2
      ],
      "name": "Wenhua Lu"
    },
    {
      "affs": [
        1
      ],
      "name": "Yizheng Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Yangyang An"
    },
    {
      "affs": [
        0
      ],
      "name": "Qihong Yan"
    },
    {
      "affs": [
        3
      ],
      "name": "Xubin Yang"
    },
    {
      "affs": [
        1,
        4
      ],
      "name": "Tiangang Luan",
      "orcid": "0000-0002-1546-0902"
    },
    {
      "affs": [
        1
      ],
      "name": "Baowei Chen",
      "orcid": "0000-0002-5230-8264"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d160d7vjc5pc0.bib",
  "cited_by": 0,
  "doi": "10.1021/envhealth.5c00614",
  "fulltext_url": "https://europepmc.org/articles/PMC13681432",
  "id": "d160d7vjc5pc0",
  "issns": [
    "2833-8278"
  ],
  "landing_url": "https://europepmc.org/article/MED/42903685",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2304|Arsenic contamination and mitigation"
    ]
  ],
  "openalex": "W7162108074",
  "pdf_url": "https://europepmc.org/articles/PMC13681432?pdf=render",
  "pmcid": "PMC13681432",
  "pmid": "42903685",
  "published": "2026-05-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/d160d7vjc5pc0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:59Z",
      "native_id": "MED:42903685",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42903685"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2304|Arsenic contamination and mitigation",
      "subfield": "environmental"
    }
  ],
  "title": "Global Analysis of Metabolic Fluxes of Glucose and Glutamine in INS‑1 Cells Exposed to Arsenite Using ¹³C‑Isotopic Tracers.",
  "title_text": "Global Analysis of Metabolic Fluxes of Glucose and Glutamine in INS‑1 Cells Exposed to Arsenite Using ¹³C‑Isotopic Tracers.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:59Z",
  "url": "https://research.draconitic.com/paper/d160d7vjc5pc0",
  "venue": "Environment & health (Washington, D.C.)",
  "versions": []
}