{
  "abstract": "Reliable plasma cell-free DNA extraction is a key component of liquid biopsy testing and requires standardized preanalytical workflows. We conducted a paired comparison of 2 automated extraction methods, the Maxwell RSC ccfDNA LV Plasma Kit and the Maxwell RSC Rapid ccfDNA Kit, using EDTA plasma samples from 48 patients with early-stage colorectal cancer. For each patient, 4 mL of plasma was divided into two 2 mL aliquots and processed on the same instrument according to the manufacturer’s instructions. Cell-free DNA was measured by fluorometry and capillary electrophoresis, and outcomes were analyzed using linear mixed-effects models with extraction kit as a fixed effect and patient as a random intercept. Measurements obtained by the two analytical platforms were strongly correlated. Compared with the Standard workflow, the Rapid workflow showed significantly higher values across all evaluated concentration and fragment-distribution endpoints, including total DNA concentration and fragment recovery in the 50–700 bp and 100–300 bp ranges. In this early-stage colorectal cancer cohort, the Rapid workflow showed higher cfDNA recovery and a greater representation of fragments within the evaluated cfDNA size ranges than the Standard workflow. These findings are limited to upstream extraction and quality-control metrics and do not establish improved mutation detection, analytical sensitivity, or suitability for downstream molecular assays. The findings indicate that this simplified workflow may be a practical alternative for routine plasma cell-free DNA extraction, although local analytical verification remains advisable.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Valeria Denninghoff"
    },
    {
      "name": "Inés Aznar-Peralta"
    },
    {
      "name": "Cristóbal Fresno"
    },
    {
      "name": "Álvaro Mellado-Cuesta"
    },
    {
      "name": "Simona Innamorato"
    },
    {
      "name": "María Pilar Molina-Vallejos"
    },
    {
      "name": "Javier López-Hidalgo"
    },
    {
      "name": "María José Serrano"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d1cgq7fhfww5z.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-69239-2",
  "fulltext_url": null,
  "id": "d1cgq7fhfww5z",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-69239-2",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|Cancer Genomics and Diagnostics"
    ]
  ],
  "openalex": "W7223900992",
  "pdf_url": "https://www.nature.com/articles/s41598-026-69239-2.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-27",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/d1cgq7fhfww5z.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:34Z",
      "native_id": "10.1038/s41598-026-69239-2",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-69239-2"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Cancer Genomics and Diagnostics",
      "subfield": "oncology"
    }
  ],
  "title": "Comparative evaluation of two automated magnetic-bead cfDNA extraction workflows in early colorectal cancer",
  "title_text": "Comparative evaluation of two automated magnetic-bead cfDNA extraction workflows in early colorectal cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:34Z",
  "url": "https://research.draconitic.com/paper/d1cgq7fhfww5z",
  "venue": "Scientific Reports",
  "versions": []
}