{
  "abstract": "Dietary magnesium (Mg2+) supplement enhances memory in mammals and Drosophila, but whether and how Mg2+ changes molecular states of neurons, remains unknown. Here we used single-cell RNA sequencing to map cell-type specific transcriptional responses to memory-enhancing dietary Mg2+. These analyses revealed changes in Hippo-pathway gene expression in memory-relevant {beta} Kenyon Cells (KCs) of the mushroom bodies. Targeted knock-down of Merlin, hippo (hpo) and warts demonstrated requirement in {beta} KCs for Mg2+-enhanced, but not baseline memory. Further study of Hippo signaling identified Yorkie (yki) transcriptional coactivator and DNA-binding partner Scalloped (sd) to be required for baseline and Mg2+-enhanced memory. Moreover, releasing Yki::Sd of inhibition from Tondu-domain containing growth inhibitor (Tgi) specifically in {beta} KCs enhanced memory, independent of diet. Analyses of Yki::Sd target genes identified several required in {beta} KCs for baseline memory, including crb and Glut1 which are also Mg2+-induced in a Hippo-dependent manner in {beta} KCs. These genes suggest that aiding the metabolic demands of long-term memory and facilitating structural neuronal plasticity are key mediators of Mg2+-induced memory enhancement. We propose pharmacological enhancement of Sd-dependent transcription, and activity of particular Sd-target genes may have therapeutic potential for memory disorders.",
  "abstract_shown": true,
  "affiliations": [
    "University of Oxford"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Y. Wu"
    },
    {
      "name": "C. D. Treiber"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Waddell"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d2dw1mkgv1nzp.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.02.756214",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756214v1.full",
  "id": "d2dw1mkgv1nzp",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756214v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ],
    [
      "openalex",
      "2804|Neurobiology and Insect Physiology Research"
    ]
  ],
  "openalex": "W7221173469",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756214v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-02",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/d2dw1mkgv1nzp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:42Z",
      "native_id": "10.64898/2026.10.02.756214",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756214v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "Dietary magnesium supplement enhances memory through Hippo signaling",
  "title_text": "Dietary magnesium supplement enhances memory through Hippo signaling",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:42Z",
  "url": "https://research.draconitic.com/paper/d2dw1mkgv1nzp",
  "venue": "bioRxiv",
  "versions": []
}