{
  "abstract": "L-lysine stands out as a commercially important amino acid vital for maintaining balanced nutrition, immune function and healthy development in humans and animals. As global demand for lysine continues to rise each year, large-scale production is required to keep pace with this growing need. In the present study, L-lysine production was accomplished through a fermentation process utilizing isolated bacterial strains from soil. After conducting primary screening, Bacillus megaterium NTJT4 was identified as the highest lysine producer. Various fermentation parameters including fermentation time, pH, temperature, different carbon and nitrogen sources, glucose levels, ammonium sulfate concentration, growth stimulators, and vitamin concentrations were evaluated to optimize lysine production. The study found that the optimal conditions for lysine production were 72 hours, pH 7.0, and 37 °C. The effect of different carbon and nitrogen sources showed that 8% glucose and 1% ammonium sulfate resulted in increased lysine production (4.86 ± 0.20 mg/mL and 3.95 ± 0.14 mg/mL respectively). Media with combination of peptone and yeast extract as growth stimulators showed increased lysine yield (4.86 ± 0.31 mg/mL) and addition of 100 µg/mL thiamine-HCL as a source of vitamin resulted in increased lysine yield (5.37 ± 0.16 mg/mL). Amberlite IR‑120–based ion‑exchange chromatography achieved 100.0% purity and 58.0% recovery of lysine. The purified lysine concentration reached 13.44 mg/mL with a purification fold of 3.85. These results indicate that the newly isolated Bacillus megaterium NTJT4 possesses considerable potential for diverse biotechnological and industrial applications, particularly in microbial amino acid production.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Taslimul Jannat"
    },
    {
      "affs": [
        0
      ],
      "name": "Md Rezaul Karim"
    },
    {
      "affs": [
        0
      ],
      "name": "Md Rakibul Hasan"
    },
    {
      "affs": [
        0
      ],
      "name": "Debabrata Karmakar"
    },
    {
      "affs": [
        0
      ],
      "name": "Nishat Tasnim"
    },
    {
      "affs": [
        0
      ],
      "name": "Nourin Tarannum",
      "orcid": "0000-0001-5912-8824"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d2ed48cm1nyje.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0361763",
  "fulltext_url": "https://europepmc.org/articles/PMC13722322",
  "id": "d2ed48cm1nyje",
  "issns": [
    "1932-6203"
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  "landing_url": "https://doi.org/10.1371/journal.pone.0361763",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Amino Acid Analysis"
    ],
    [
      "heading",
      "Amino Acids"
    ],
    [
      "heading",
      "Bacillus"
    ],
    [
      "heading",
      "Bacteria"
    ],
    [
      "heading",
      "Bacterial Pathogens"
    ],
    [
      "heading",
      "Basic Amino Acids"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Biology and life sciences"
    ],
    [
      "heading",
      "Carbohydrates"
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    [
      "heading",
      "Cell biology"
    ],
    [
      "heading",
      "Cellular structures and organelles"
    ],
    [
      "heading",
      "Chemical Compounds"
    ],
    [
      "heading",
      "Chemistry"
    ],
    [
      "heading",
      "Fermentation"
    ],
    [
      "heading",
      "Glucose"
    ],
    [
      "heading",
      "Lysine"
    ],
    [
      "heading",
      "Medical Microbiology"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Metabolic Processes"
    ],
    [
      "heading",
      "Metabolism"
    ],
    [
      "heading",
      "Microbial Pathogens"
    ],
    [
      "heading",
      "Microbiology"
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    [
      "heading",
      "Molecular Biology"
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    [
      "heading",
      "Molecular Biology Assays and Analysis Techniques"
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    [
      "heading",
      "Molecular Biology Techniques"
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      "heading",
      "Monosaccharides"
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    [
      "heading",
      "Non-coding RNA"
    ],
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      "heading",
      "Nucleic acids"
    ],
    [
      "heading",
      "Organic Chemistry"
    ],
    [
      "heading",
      "Organic Compounds"
    ],
    [
      "heading",
      "Organisms"
    ],
    [
      "heading",
      "Pathogens"
    ],
    [
      "heading",
      "Pathology and Laboratory Medicine"
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    [
      "heading",
      "Physical Sciences"
    ],
    [
      "heading",
      "Proteins"
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      "heading",
      "RNA"
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    [
      "heading",
      "Research Article"
    ],
    [
      "heading",
      "Research and Analysis Methods"
    ],
    [
      "heading",
      "Ribosomal RNA"
    ],
    [
      "heading",
      "Ribosomes"
    ],
    [
      "heading",
      "Salts"
    ],
    [
      "heading",
      "Sulfates"
    ],
    [
      "heading",
      "Vitamins"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Metabolism/Metabolic processes/Fermentation"
    ],
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      "plos",
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    ],
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      "plos",
      "/Physical sciences/Chemistry/Chemical compounds/Organic compounds/Amino acids/Basic amino acids/Lysine"
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      "plos",
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    [
      "plos",
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    [
      "plos",
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    [
      "plos",
      "/Physical sciences/Chemistry/Organic chemistry/Organic compounds/Amino acids/Basic amino acids/Lysine"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Organic chemistry/Organic compounds/Carbohydrates/Monosaccharides/Glucose"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Organic chemistry/Organic compounds/Vitamins"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Molecular biology assays and analysis techniques/Amino acid analysis"
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  "pdf_url": "https://europepmc.org/articles/PMC13722322?pdf=render",
  "pmcid": "PMC13722322",
  "pmid": "42961983",
  "published": "2026-10-30",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/d2ed48cm1nyje.ris",
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      "first_seen": "2026-11-03T12:22:51Z",
      "native_id": "PMC13722322",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Metabolism",
      "subfield": "enzymology-metabolism"
    },
    {
      "field": "biology",
      "origin": "heading:Cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "heading:Microbiology",
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  "title": "Isolation to purification: An integrated study on lysine production by Bacillus megaterium NTJT4 from medicinal plant rhizosphere",
  "title_text": "Isolation to purification: An integrated study on lysine production by Bacillus megaterium NTJT4 from medicinal plant rhizosphere",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:51Z",
  "url": "https://research.draconitic.com/paper/d2ed48cm1nyje",
  "venue": "PLOS One",
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}