{
  "abstract": "农药残留对生态系统安全和人类健康构成持续威胁，亟需发展兼具高灵敏度与便携性的快速检测技术。酶生物传感器虽具备优异的分子识别能力，但其实际应用常受限于天然酶固有的稳定性问题。多孔框架材料，包括金属有机框架、共价有机框架与氢键有机框架材料，凭借其超高比表面积、可调的孔道结构及良好的化学稳定性为酶的固定化提供了理想载体，可显著提升酶的催化活性、操作稳定性及底物选择性。本文系统综述了基于多孔框架材料构建的固定化酶生物传感器在农药监测中的研究进展。首先，归纳了表面吸附、后渗透与原位固定3类酶固定化方法，探讨了其在负载效率、稳定性及选择性方面的特征与适用性；进而，详细阐述了基于比色、荧光、电化学及化学发光等信号转导机制的传感模式，分析了其响应原理与检测性能。最后，针对复杂基质耐受性、多残留同步识别、绿色合成以及智能传感系统集成等现场应用中面临的关键挑战，提出了未来发展方向，旨在为开发高可靠性、适应复杂场景的农药快速检测技术提供理论依据与设计参考。",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "review article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Zhengyu LAN"
    },
    {
      "name": "Xiaoxue KOU"
    },
    {
      "name": "Jing LI"
    },
    {
      "name": "Tianyi WEN"
    },
    {
      "name": "Rui GAO"
    },
    {
      "name": "Siming HUANG"
    },
    {
      "name": "Guosheng CHEN"
    },
    {
      "name": "Gangfeng OUYANG"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d2nva04dn32qv.bib",
  "cited_by": 0,
  "doi": "10.3724/sp.j.1123.2026.03022",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13729052/",
  "id": "d2nva04dn32qv",
  "issns": [
    "1000-8713",
    "1872-2059"
  ],
  "landing_url": "https://doi.org/10.3724/sp.j.1123.2026.03022",
  "language": "zh",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "O658"
    ],
    [
      "heading",
      "Reviews"
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    [
      "heading",
      "a"
    ],
    [
      "openalex",
      "1312|Enzyme Catalysis and Immobilization"
    ]
  ],
  "openalex": "W7225190764",
  "pdf_url": null,
  "pmcid": "PMC13729052",
  "pmid": "42973222",
  "published": "2026-11-07",
  "publisher": "Editorial Office of Chinese Journal of Chromatography",
  "ris": "https://research.draconitic.com/paper/d2nva04dn32qv.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T12:21:53Z",
      "native_id": "PMC13729052",
      "source": "pmc",
      "url": "https://doi.org/10.3724/sp.j.1123.2026.03022"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Enzyme Catalysis and Immobilization",
      "subfield": "molecular-biology"
    }
  ],
  "title": "多孔框架材料固定化酶生物传感器用于农药残留分析的研究进展",
  "title_text": "多孔框架材料固定化酶生物传感器用于农药残留分析的研究进展",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:53Z",
  "url": "https://research.draconitic.com/paper/d2nva04dn32qv",
  "venue": "Chinese Journal of Chromatography",
  "versions": []
}