{
  "abstract": "FSFC is an emerging technology that can greatly enhance our understanding of the single-cell proteomic landscape. We demonstrate that this application is applicable to cells derived from solid tissues which have traditionally been hampered by their complex autofluorescence signatures and lack of optimized tools for non-immune cells. We present a protocol and discuss key controls that minimize the impact of unmixing errors, enabling us to resolve multiple EC subpopulations isolated from different tissues in models of chronic tissue injury.\n\nResearch topic(s): vascular biology, cell heterogeneity, full spectrum flow cytometry.",
  "abstract_shown": true,
  "affiliations": [
    "Centre for Microvascular Research, William Harvey Research Institute, Queen Mary University of London, EC1M 6BQ, United Kingdom",
    "Flow Cytometry Core Facility, Barts Cancer Institute, Queen Mary University of London, London, EC1M 6BQ, United Kingdom"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Christina Gkantsinikoudi"
    },
    {
      "affs": [
        1
      ],
      "name": "Manuela Terranova-Barberio"
    },
    {
      "affs": [
        0
      ],
      "name": "Neil Dufton"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d4g329jjczz2c.bib",
  "cited_by": 0,
  "doi": "10.1016/j.bbrep.2026.102845",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13696768/",
  "id": "d4g329jjczz2c",
  "issns": [
    "2405-5808"
  ],
  "landing_url": "https://doi.org/10.1016/j.bbrep.2026.102845",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Short Communication"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7222809387",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13696768/pdf/?tool=EBI",
  "pmcid": "PMC13696768",
  "pmid": "42935706",
  "published": "2026-10-17",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/d4g329jjczz2c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:04Z",
      "native_id": "PMC13696768",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.bbrep.2026.102845"
    },
    {
      "first_seen": "2026-10-30T12:21:25Z",
      "native_id": "PMC:PMC13696768",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13696768"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells",
  "title_text": "Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:03Z",
  "url": "https://research.draconitic.com/paper/d4g329jjczz2c",
  "venue": "Biochemistry and Biophysics Reports",
  "versions": []
}