{
  "abstract": "We examined Kv1.3 expression and function in articular cartilage and primary chondrocytes from male Wistar rats using reverse transcription polymerase chain reaction (RT-PCR), Western blotting, immunofluorescence, and whole-cell patch-clamp recordings. Molecular assays supported Kcna3 transcript and Kv1.3 protein expression. PAP-1 and ShK-186 revealed blocker-sensitive outward currents, depolarized freshly isolated chondrocytes, and increased input resistance. During primary culture, total and PAP-1-sensitive conductance densities decreased, without a statistically significant change in the relative PAP-1-sensitive contribution. In unadjusted paired analyses, IL-1α and IL-1β increased PAP-1-sensitive conductance; IL-1β also reduced total conductance and increased the relative contribution. The relative increase with IL-1α was sensitive to the statistical approach. No statistically significant conductance changes were detected after LPS exposure. Blocker effects varied by cytokine and endpoint: PAP-1 reduced propidium iodide (PI) positivity following IL-1α exposure, whereas both blockers attenuated IL-1β-associated changes in metabolic activity, PI positivity, and cell density in paired analyses; the ShK-186 metabolic effect was sensitive to the statistical approach. These findings support a functional contribution of Kv1.3-sensitive conductance to chondrocyte electrical properties and responses to inflammatory stress.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Physiology, Biophysics and Neurosciences, Centro de Investigacion y Estudios Avanzados del IPN, Ciudad de México 07360, Mexico"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Arturo Ponce",
      "orcid": "0000-0002-2575-5210"
    },
    {
      "affs": [
        0
      ],
      "name": "Lorena Hinojosa"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria Luisa Roldán"
    },
    {
      "affs": [
        0
      ],
      "name": "Liora Shoshani",
      "orcid": "0000-0002-3442-4333"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d6end4f2pfdad.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219790",
  "fulltext_url": null,
  "id": "d6end4f2pfdad",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9790",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-31",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/d6end4f2pfdad.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:20:58Z",
      "native_id": "10.3390/ijms27219790",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9790"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Molecular and Functional Characterization of Kv1.3 Channels in Rat Articular Cartilage and Primary Chondrocytes: Regulation During Culture and Pro-Inflammatory Stimulation",
  "title_text": "Molecular and Functional Characterization of Kv1.3 Channels in Rat Articular Cartilage and Primary Chondrocytes: Regulation During Culture and Pro-Inflammatory Stimulation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T00:21:14Z",
  "url": "https://research.draconitic.com/paper/d6end4f2pfdad",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}