{
  "abstract": "Introduction: Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related mortality, particularly due to resistance induced by tumor-associated macrophages (TAMs)-exhibiting a M2-like phenotype-within the tumor microenvironment. The specific targeting of pro-tumoral M2 TAMs constitutes thus a major challenge in anticancer therapies but current strategies lack specificity. We produced and patented a monoclonal antibody, called mAb6-25, that specifically targets M2-like macrophages (M2M) but not M1-like macrophages (M1M) or monocytes. Here, we investigated the conjugation of this antibody on magnetic nanoparticles (MNPs) as a potential nanoplatform for specific M2 TAM targeting. Methods: Magnetic iron oxide multicore nanoflowers (NF) were functionalized with a fluorophore allowing their detection and the mAb6-25 for M2 TAM targeting. The M2M targeting by MNP-mAb6-25 was determined, comparatively to M1M, in 2D and 3D in vitro co-culture models with cancer cells by flow cytometry and confocal microscopy analysis, while MNP-mAb6-25 uptake was evaluated by NMR relaxometry. The in vivo M2 TAM targeting was analyzed in a xenograft mouse model of NSCLC by IVIS optical imaging.\n\nResults:We demonstrated that the MNP-mAb6-25 nanoplatform preferentially binds M2M compared to M1M (13-fold higher uptake in M2M) or cancer cells in monoculture as well as in 2D and 3D co-culture models mixing M2M or M1M with A549 NSCLC cells. Moreover, MNP-mAb6-25 internalized and accumulated in the lysosomes of M2M. The efficacy of intravenously injected MNP-mAb6-25 to target NSCLC sub-cutaneous xenografted models containing M2M was subsequently demonstrated.\n\nConclusion:This study highlights the potential of MNP-mAb6-25 as a nanoplatform for selective targeting TAM-containing NSCLC tumors. This nanotechnology tool may lead to the development of new applications: one involving the imaging-based detection of protumoral M2 TAMs by MRI, and the other involving their potential depletion through the application of localized magnetic fields, paving the way for imaging-guided diagnosis and further therapeutic evaluation.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Chloe Bazile"
    },
    {
      "name": "Julie Bordenave"
    },
    {
      "name": "Saber Amri"
    },
    {
      "name": "Flavien Raynal"
    },
    {
      "name": "Laetitia Ligat"
    },
    {
      "name": "Manon Farcé"
    },
    {
      "name": "Henri Coste"
    },
    {
      "name": "Pascal Clerc"
    },
    {
      "name": "Olivier Sandre"
    },
    {
      "name": "Corinne Bousquet"
    },
    {
      "name": "Vera Pancaldi"
    },
    {
      "name": "Stéphane Mornet"
    },
    {
      "name": "Veronique Gigoux"
    },
    {
      "name": "Mary Poupot"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d6kgwex3m3nxr.bib",
  "cited_by": 0,
  "doi": "10.2147/ijn.s619574",
  "fulltext_url": null,
  "id": "d6kgwex3m3nxr",
  "issns": [
    "1176-9114",
    "1178-2013"
  ],
  "landing_url": "https://u-bordeaux.hal.science/hal-05765929v1",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.ib"
    ],
    [
      "hal",
      "1.sdv.imm"
    ],
    [
      "hal",
      "2.sdv.ib.bio"
    ],
    [
      "hal",
      "2.sdv.imm.imm"
    ],
    [
      "openalex",
      "2403|Immune cells in cancer"
    ]
  ],
  "openalex": "W7204694200",
  "pdf_url": "https://u-bordeaux.hal.science/hal-05765929/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-29",
  "publisher": "Dove Medical Press",
  "ris": "https://research.draconitic.com/paper/d6kgwex3m3nxr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:41Z",
      "native_id": "hal-05765929",
      "source": "hal",
      "url": "https://u-bordeaux.hal.science/hal-05765929v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.imm",
      "subfield": "immunology"
    },
    {
      "field": "biology",
      "origin": "hal:1.sdv.ib",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Precision Targeting of Human M2 Macrophages by Magnetic Nanoparticles Functionalized with an in-House Antibody",
  "title_text": "Precision Targeting of Human M2 Macrophages by Magnetic Nanoparticles Functionalized with an in-House Antibody",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:41Z",
  "url": "https://research.draconitic.com/paper/d6kgwex3m3nxr",
  "venue": "International Journal of Nanomedicine",
  "versions": []
}