{
  "abstract": "Atherosclerosis (AS), a disease associated with aging, significantly compromises the health and quality of life of patients. β-galactosidase (β-gal), a key cell senescence biomarker, shows significantly increased activity in senescent cells. Upregulated β-gal in senescent cells amplifies a proinflammatory senescence-associated secretory phenotype (SASP), driving cellular dysfunction, monocyte recruitment, foam cell formation, and extracellular matrix remodeling, thereby promoting atherogenesis. Inhibiting β-gal activity is a potential strategy for delaying AS and other aging-related diseases. Herein, we designed and synthesized a novel near-infrared fluorescent probe activated by β-gal (NFP-gal). This probe exhibited high sensitivity and selectivity with low cytotoxicity and biotoxicity under the tested conditions. We used this fluorescence-guided screening method to evaluate the β-gal inhibitory activity of 58 traditional Chinese medicines (TCMs) and identified Arnebia euchroma (AE) as a strong candidate. Through targeted separation and identification of complex components in AE using ultra-high-performance liquid chromatography with quadrupole orbitrap high-resolution mass spectrometry (UHPLC-Q-orbitrap-HRMS), we identified β,β-dimethyl-acryl-alkannin (DMAA) as a novel β-gal inhibitor. The pharmacodynamic effects of this compound were validated through in vitro cellular imaging using senescent cell and foam cell models, real-time in vivo fluorescence tracking in zebrafish models of AS, and histopathological analysis using apolipoprotein E-deficient (ApoE -/-) mice with high-fat diet (HFD)-induced AS. The findings confirmed the therapeutic potential of DMAA in mitigating senescence-associated vascular dysfunction. This study comprehensively evaluated the use of β-gal-based probes and natural compounds to suppress aging-related processes, highlighting their therapeutic implications for AS and other aging-associated diseases.",
  "abstract_shown": true,
  "affiliations": [
    "School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.",
    "Beijing Hton Co., Ltd., Beijing, 100010, China.",
    "Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.",
    "College of Veterinary Medicine, Yunnan Agricultural University, Yunan, 650500, China.",
    "Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.",
    "Shandong Key Laboratory of Digital Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Yujuan Qin"
    },
    {
      "affs": [
        2
      ],
      "name": "Ning Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Wenjie Yang"
    },
    {
      "affs": [
        3
      ],
      "name": "Yi Wu"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Zhixin Tang"
    },
    {
      "affs": [
        2,
        5
      ],
      "name": "Junhong Xin"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Haiqiang Jiang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/d6xxzmka7mzwf.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jpha.2026.101659",
  "fulltext_url": "https://europepmc.org/articles/PMC13683445",
  "id": "d6xxzmka7mzwf",
  "issns": [
    "2095-1779",
    "2214-0883"
  ],
  "landing_url": "https://europepmc.org/article/MED/42908895",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Molecular Sensors and Ion Detection"
    ]
  ],
  "openalex": "W7161233738",
  "pdf_url": "https://europepmc.org/articles/PMC13683445?pdf=render",
  "pmcid": "PMC13683445",
  "pmid": "42908895",
  "published": "2026-05-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/d6xxzmka7mzwf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:14Z",
      "native_id": "MED:42908895",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42908895"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Molecular Sensors and Ion Detection",
      "subfield": "physical"
    }
  ],
  "title": "Near-infrared fluorescent probe-mediated high-throughput discovery of a natural β-galactosidase inhibitor for senescence-associated atherosclerosis therapy.",
  "title_text": "Near-infrared fluorescent probe-mediated high-throughput discovery of a natural β-galactosidase inhibitor for senescence-associated atherosclerosis therapy.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:14Z",
  "url": "https://research.draconitic.com/paper/d6xxzmka7mzwf",
  "venue": "Journal of pharmaceutical analysis",
  "versions": []
}