{
  "abstract": "Multiple lines of evidence suggest that RNase H1-sensitive RNA:DNA hybrids could disrupt the stability and restart of stalled replication forks. The prolonged over-expression of RNase H1 is the most popular method to manipulate RNA:DNA hybrids in situ but it cannot discriminate between direct and indirect effects resulting from their loss. To overcome this limitation, we developed iCoRD (in Cellulo RNase H Delivery) to rapidly deliver comparable amounts of either Escherichia coli RNase H1 (RnhA) or control proteins in live cells, with a negligible impact on the transcriptome. iCoRD-delivered RnhA recognises RNA:DNA hybrids in situ, is enriched in the vicinity of active replication forks and rescues replication fork progression in different stress conditions. While iCoRD-delivered RnhA had a clear impact on stress-induced, fork-proximal RNA:DNA hybrids and R-ChIP signals, it had no effect on DRIP or S9.6 Cut&Tag signals, even when these were induced by stress. Our results strongly suggest the existence of distinct populations of RNA:DNA hybrids that are best mapped by different methods and are differently sensitive to RNase H1 activity in situ.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Amandine Basille"
    },
    {
      "name": "Yara Bawadi"
    },
    {
      "name": "Yea-Lih Lin"
    },
    {
      "name": "Connor Kirke"
    },
    {
      "name": "Clara Bourgeay"
    },
    {
      "name": "David Cluet"
    },
    {
      "name": "Laetitia Vachez"
    },
    {
      "name": "Virgile Dervieux"
    },
    {
      "name": "Laura Guiguettaz"
    },
    {
      "name": "Pascal Bernard"
    },
    {
      "name": "Stephan Hamperl"
    },
    {
      "name": "Philippe Pasero"
    },
    {
      "name": "Emiliano Ricci"
    },
    {
      "name": "Vincent Vanoosthuyse"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dan63j6anbwm3.bib",
  "cited_by": 0,
  "doi": "10.1038/s44318-026-00949-z",
  "fulltext_url": null,
  "id": "dan63j6anbwm3",
  "issns": [
    "0261-4189",
    "1460-2075"
  ],
  "landing_url": "https://hal.science/hal-05843318v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
    ],
    [
      "hal",
      "2.sdv.bbm.bm"
    ],
    [
      "openalex",
      "1312|DNA Repair Mechanisms"
    ]
  ],
  "openalex": "W7223099450",
  "pdf_url": "https://link.springer.com/content/pdf/10.1038/s44318-026-00949-z.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-22",
  "publisher": "Springer nature",
  "ris": "https://research.draconitic.com/paper/dan63j6anbwm3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:03Z",
      "native_id": "hal-05843318",
      "source": "hal",
      "url": "https://hal.science/hal-05843318v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.bm",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "The rapid delivery of RNase H variants reveals distinct classes of RNA:DNA hybrids",
  "title_text": "The rapid delivery of RNase H variants reveals distinct classes of RNA:DNA hybrids",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:20:56Z",
  "url": "https://research.draconitic.com/paper/dan63j6anbwm3",
  "venue": "The EMBO Journal",
  "versions": []
}