{
  "abstract": "This study established a surface-enhanced Raman scattering (SERS) detection method based on the catalytic hairpin assembly (CHA) reaction combined with clustered regularly interspaced short palindromic repeats and its associated proteins (CRISPR/Cas), integrated with the guanine-quadruplexes/Hemin (G4/Hemin) catalytic amplification effect, which is available for identifying a typical indicator for genetically modified crops, the 35S promoter from the cauliflower mosaic virus (CaMV35S). Target CaMV35S-derived RNA (CaMV35S RNA) triggers the CHA reaction, yielding a double-stranded DNA product (C1/C2) that is recognized by CRISPR RNA (crRNA), thereby activating the trans-cleavage activity of Cas12a. A magnetic bead-based nanoprobe was fabricated by linking the hybridization chain reaction (HCR) product to the magnetic bead. This probe can fold into repeated G4 units under a specific potassium ion concentration to enable a high loading of Hemin, thereby providing strong peroxidase-like catalytic activity for 3,3',5,5'-tetra-methyl-benz-idine (TMB). In a positive trial, the 35S RNA initiates CHA, and the CHA product activates Cas12a, disrupting the HCR products on the magnetic bead probe, preventing the G4 structure formation, accordingly yielding a weak SERS signal from TMB. In contrast, a negative trial fails to activate Cas12a, and the probe retains its nanoenzyme activity, resulting in a strong SERS signal from the produced oxTMB. Owing to the collateral trans-cleavage activity of Cas12a and the high peroxidase-like nanozyme activity of G4/Hemin, this method achieves the highly sensitive SERS detection of genetically modified samples without target gene preamplification.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.",
    "School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, P. R. China.",
    "Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Changchun 130033, P. R. China.",
    "Maize Research Institute, Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Changchun 130118, P.R. China.",
    "Center for Supramolecular Chemical Biology, College of Chemistry, Jilin University, Changchun 130012, P. R. China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Lin Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Chengxin Bao"
    },
    {
      "affs": [
        0
      ],
      "name": "Huimin Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Feiwu Li"
    },
    {
      "affs": [
        3
      ],
      "name": "Xiangguo Liu"
    },
    {
      "affs": [
        2
      ],
      "name": "Yuxuan He"
    },
    {
      "affs": [
        1
      ],
      "name": "Jingjing Chang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yuejia Yin"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Shuping Xu",
      "orcid": "0000-0002-6216-6175"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dbkxsc704zcqp.bib",
  "cited_by": 0,
  "doi": "10.1021/acsmeasuresciau.6c00142",
  "fulltext_url": "https://europepmc.org/articles/PMC13693178",
  "id": "dbkxsc704zcqp",
  "issns": [
    "2694-250X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42921428",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Advanced biosensing and bioanalysis techniques"
    ]
  ],
  "openalex": "W7204035629",
  "pdf_url": "https://europepmc.org/articles/PMC13693178?pdf=render",
  "pmcid": "PMC13693178",
  "pmid": "42921428",
  "published": "2026-08-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dbkxsc704zcqp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:02Z",
      "native_id": "MED:42921428",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42921428"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Advanced biosensing and bioanalysis techniques",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Catalytic Hairpin Assembly-Aided CRISPR/Cas-SERS for Nucleic Acid Detection Enhanced by Multiple Catalytic Amplification.",
  "title_text": "Catalytic Hairpin Assembly-Aided CRISPR/Cas-SERS for Nucleic Acid Detection Enhanced by Multiple Catalytic Amplification.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:02Z",
  "url": "https://research.draconitic.com/paper/dbkxsc704zcqp",
  "venue": "ACS measurement science au",
  "versions": []
}