{
  "abstract": "Constitutive heterochromatin, characterized by histone H3 lysine 9 trimethylation (H3K9me3), is essential for genome stability, pluripotency, and developmental fidelity. While the SUV39H histone methyltransferases catalyze H3K9me3 deposition, their locus-specific targeting mechanisms remain unclear. Here, via RNA depletion or RNA-binding affinity mutation, we establish that RNA binding is indispensable for SUV39H2 recruitment to chromatin and H3K9me3 maintenance in mouse embryonic stem cells (ESCs). Through RNA immunoprecipitation sequencing (RIP-Seq) and functional validation, we identify the long non-coding RNA Gas5 as a specific SUV39H2 interactor. Depletion of Gas5 or disruption of the SUV39H2–RNA interaction leads to the loss of self-renewal capacity of ESCs, abolishes H3K9me3 enrichment, along with profound genomic instability, mitotic errors, and γH2AX foci accumulation. These findings reveal a critical lncRNA-dependent mechanism governing the SUV39H2–H3K9me3 axis, which directly couples RNA metabolism to the preservation of pluripotency and genome integrity in stem cells.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Juntao Liu"
    },
    {
      "name": "Jiani Yang"
    },
    {
      "name": "Wen Ye"
    },
    {
      "name": "Dandan Zhang"
    },
    {
      "name": "Nan Su"
    },
    {
      "name": "Hong Wang"
    },
    {
      "name": "Yanping Zhang",
      "orcid": "0000-0003-3565-8120"
    },
    {
      "name": "Shaorong Gao",
      "orcid": "0000-0003-1041-3928"
    },
    {
      "name": "Lan Kang",
      "orcid": "0000-0003-4525-5923"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dbnrtccnajmbn.bib",
  "cited_by": 0,
  "doi": "10.1093/procel/pwag039",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13721125/",
  "id": "dbnrtccnajmbn",
  "issns": [
    "1674-800X",
    "1674-8018"
  ],
  "landing_url": "https://doi.org/10.1093/procel/pwag039",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "AcademicSubjects/SCI00960"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1306|Cancer-related molecular mechanisms research"
    ]
  ],
  "openalex": "W7165628640",
  "pdf_url": null,
  "pmcid": "PMC13721125",
  "pmid": "42334949",
  "published": "2026-06-23",
  "publisher": "Oxford University Press",
  "ris": "https://research.draconitic.com/paper/dbnrtccnajmbn.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:21:52Z",
      "native_id": "PMC13721125",
      "source": "pmc",
      "url": "https://doi.org/10.1093/procel/pwag039"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Cancer-related molecular mechanisms research",
      "subfield": "oncology"
    }
  ],
  "title": "LncRNA Gas5 directs SUV39H2 to establish heterochromatin and maintain genome stability",
  "title_text": "LncRNA Gas5 directs SUV39H2 to establish heterochromatin and maintain genome stability",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:21:52Z",
  "url": "https://research.draconitic.com/paper/dbnrtccnajmbn",
  "venue": "Protein & Cell",
  "versions": []
}