{
  "abstract": "Burkholderia cenocepacia is a problematic pathogen that infects people with cystic fibrosis and can cause fatal \"cepacia syndrome\". B. cenocepacia infection is difficult to treat due to the high-level intrinsic resistance of the bacterium to antimicrobials and its ability to survive in macrophages. In this study, we uncover a hitherto unknown aspect of B. cenocepacia's pathogenesis related to the formation of new glyceroglycolipids, which are involved in intracellular survival. Using lipidomics, we observed that B. cenocepacia can produce three glyceroglycolipid species in phosphate deplete conditions using a PlcP-mediated lipid remodeling pathway originally discovered in soil and ocean-dwelling bacteria. While lipid remodeling as an adaptive strategy for environmental microbes to overcome phosphorus limitation is known, its role in intracellular bacterial survival has not been investigated. Using mammalian macrophages and Galleria mellonella larvae as infection models, we show that mutants unable to perform membrane lipid remodeling (ΔplcP, Δagt) could not establish an infection. Unlike the wild-type bacterium, the ΔplcP mutant does not replicate within macrophages. Comparative genomics analyses showed that this PlcP-Agt pathway is conserved in all pathogenic Burkholderia that infect mammalian and plant hosts. Overall, our results indicate that bacterial membrane lipid remodeling plays an essential role in the intracellular survival of B. cenocepacia.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Holly Shropshire"
    },
    {
      "name": "Xu Han"
    },
    {
      "name": "Zengsheng Han"
    },
    {
      "name": "Barbara Clough"
    },
    {
      "name": "Rebekah Jones"
    },
    {
      "name": "Shadman Ahmed"
    },
    {
      "name": "Inmaculada García-Romero"
    },
    {
      "name": "Isabel Aberdeen"
    },
    {
      "name": "Prachetash Ghosh"
    },
    {
      "name": "Ioannis Nezis"
    },
    {
      "name": "Miguel Valvano"
    },
    {
      "name": "David Scanlan"
    },
    {
      "name": "Richard Guillonneau"
    },
    {
      "name": "Yin Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dbrhagw0yje7j.bib",
  "cited_by": 1,
  "doi": "10.1016/j.jbc.2026.113600",
  "fulltext_url": null,
  "id": "dbrhagw0yje7j",
  "issns": [
    "0021-9258",
    "1083-351X"
  ],
  "landing_url": "https://hal.science/hal-05765126v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2713|Burkholderia infections and melioidosis"
    ]
  ],
  "openalex": "W7214108211",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-25",
  "publisher": "American Society for Biochemistry and Molecular Biology",
  "ris": "https://research.draconitic.com/paper/dbrhagw0yje7j.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:41Z",
      "native_id": "hal-05765126",
      "source": "hal",
      "url": "https://hal.science/hal-05765126v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Burkholderia infections and melioidosis",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "The phospholipase PlcP-mediated membrane lipid remodelling is required for the intracellular survival of Burkholderia cenocepacia",
  "title_text": "The phospholipase PlcP-mediated membrane lipid remodelling is required for the intracellular survival of Burkholderia cenocepacia",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:41Z",
  "url": "https://research.draconitic.com/paper/dbrhagw0yje7j",
  "venue": "Journal of Biological Chemistry",
  "versions": []
}