{
  "abstract": "Background\n\nEpsilon-poly-L-lysine (ε-PL), an antimicrobial peptide synthesized by Streptomyces albulus through submerged fermentation, is biosynthesized via multiple metabolic pathways. This suggests that global metabolic regulation and interspecies interactions contribute significantly to enhancing ε-PL yield. Current genetic and metabolic engineering strategies, however, primarily aim at strengthening specific metabolic fluxes, without adequately coordinating the diverse physiological requirements involved in ε-PL production. Previous research has shown that interspecies stress induced by Streptomyces gilvosporeus can elicit a broad enhancement of ε-PL biosynthetic pathways. Building on this finding, the present study seeks to identify key transcriptional regulators that respond to such interspecies stress and are capable of systematically improving metabolic efficiency.Results\n\nComparative transcriptomic analysis revealed that transcription factors from the RegX3, LysR, and TetR families were significantly upregulated in S. albulus under interspecies stress. Corresponding overexpression strains (OE-RegX3, OE-LysR, OE-TetR1, and OE-TetR2) were constructed using a one-step cloning strategy, all exhibiting altered colony morphology. Among them, OE-LysR showed a 60.9% increase in ε-PL production compared to the wild type in flask cultures. When scaled to a 5-L bioreacter and stimulated with S. gilvosporeus live cells, the ε-PL titer of OE-LysR further rose to 39.6 g/L, marking a 51.1% improvement. Physiological analyses revealed that the enhanced ε-PL production was attributed to the upregulation of key biosynthetic and metabolic pathways.Conclusions\n\nLysR was identified as a key regulator that mediates interspecies stress from S. gilvosporeus to enhance ε-PL biosynthesis in S. albulus. Overexpression of LysR globally activated ε-PL-related metabolic pathways and markedly increased ε-PL production at both flask and fermenter scales, especially when combined with S. gilvosporeus stimulation. These findings offer new strategies for harnessing interspecies stress to enhance antibiotic fermentation efficiency.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China.",
    "Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China.",
    "College of Life Sciences, Huaibei Normal University, 100# Dongshan road, Huaibei, Anhui, 235000, P. R. China.",
    "Huaibei Key Laboratory for Bio-processing of Agricultural Products, School of Life Sciences, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China.",
    "Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China. xzenghsd@163.com.",
    "Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China. xzenghsd@163.com.",
    "Huaibei Key Laboratory for Bio-processing of Agricultural Products, School of Life Sciences, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China. xzenghsd@163.com.",
    "College of Life Sciences, Huaibei Normal University, 100# Dongshan road, Huaibei, Anhui, 235000, P. R. China. xzenghsd@163.com.",
    "Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China. xinbingyuex@163.com.",
    "Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Huaibei Normal University, Huaibei, Anhui, 235000, P. R. China. xinbingyuex@163.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Siying Liao"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Zhanyang Zhang"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Bingxin Chen"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Siyu Tong"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Chen Zhang"
    },
    {
      "affs": [
        0,
        1,
        3,
        2
      ],
      "name": "Huawei Zeng"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Deyin Zhao"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Mingtao Zhao"
    },
    {
      "affs": [
        4,
        5,
        6,
        7
      ],
      "name": "Xin Zeng"
    },
    {
      "affs": [
        8,
        9
      ],
      "name": "Bingyue Xin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dcn1vmssy707v.bib",
  "cited_by": 0,
  "doi": "10.1186/s12896-026-01208-z",
  "fulltext_url": "https://europepmc.org/articles/PMC13718126",
  "id": "dcn1vmssy707v",
  "issns": [
    "1472-6750"
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  "landing_url": "https://europepmc.org/article/MED/42958265",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
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      "openalex",
      "2736|Microbial Natural Products and Biosynthesis"
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  "openalex": "W7172105020",
  "pdf_url": "https://europepmc.org/articles/PMC13718126?pdf=render",
  "pmcid": "PMC13718126",
  "pmid": "42958265",
  "published": "2026-08-01",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/dcn1vmssy707v.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T00:20:47Z",
      "native_id": "MED:42958265",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42958265"
    },
    {
      "first_seen": "2026-11-02T12:22:15Z",
      "native_id": "PMC13718126",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s12896-026-01208-z"
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Microbial Natural Products and Biosynthesis",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Enhanced production of ε-Poly-L-lysine through overexpression of LysR involved in interspecies interactions.",
  "title_text": "Enhanced production of ε-Poly-L-lysine through overexpression of LysR involved in interspecies interactions.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:15Z",
  "url": "https://research.draconitic.com/paper/dcn1vmssy707v",
  "venue": "BMC biotechnology",
  "versions": []
}