{
  "abstract": "As a key biomarker of Alzheimer’s disease (AD), amyloid-β (Aβ) is frequently employed for the diagnosis of AD. Near-infrared fluorescence (NIRF) imaging is a non-invasive, low-cost route for visualizing Aβ in vivo. However, most existing Aβ NIRF probes require tedious washing steps, show modest fluorescence turn-on (<50-fold), and stain normal tissue non-specifically. In this work, by fine-tuning the amphiphilicity of a hemicyanine scaffold to place its log P within the appropriate range, we identified P3 as a wash-free NIRF probe for Aβ aggregates. P3 bound Aβ aggregates with high affinity. In vivo, P3 distinguished AD mice from wild-type mice with a 4.8-fold signal ratio, together with adequate brain uptake (1.5 ± 0.1 ID/g at 20 min) and rapid clearance. Unlike Thioflavin T, which indiscriminately labels Aβ aggregates and normal neurons, P3 specifically labeled Aβ aggregates with negligible background, which enabled wash-free imaging with a signal-to-noise ratio of 80 ± 4. This work provides a wash-free, high-affinity NIRF probe for Aβ imaging.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xueqi Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "Rundong Liang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yao Li",
      "orcid": "0009-0002-8906-8230"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiawei Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Jing Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Zheng Su"
    },
    {
      "affs": [
        0
      ],
      "name": "Yang Liu",
      "orcid": "0000-0002-9284-1944"
    },
    {
      "affs": [
        0
      ],
      "name": "Huali Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Maosheng Cheng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/de0at0d8wvw3k.bib",
  "cited_by": 0,
  "doi": "10.3390/molecules31203624",
  "fulltext_url": null,
  "id": "de0at0d8wvw3k",
  "issns": [
    "1420-3049"
  ],
  "landing_url": "https://www.mdpi.com/1420-3049/31/20/3624",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2737|Alzheimer's disease research and treatments"
    ]
  ],
  "openalex": "W7221534808",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-11",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/de0at0d8wvw3k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:42Z",
      "native_id": "10.3390/molecules31203624",
      "source": "crossref",
      "url": "https://www.mdpi.com/1420-3049/31/20/3624"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Alzheimer's disease research and treatments",
      "subfield": "physiology"
    }
  ],
  "title": "Hemicyanine-Based Wash-Free Near-Infrared Fluorescent Probe for Imaging Amyloid-β Aggregates in Alzheimer’s Disease",
  "title_text": "Hemicyanine-Based Wash-Free Near-Infrared Fluorescent Probe for Imaging Amyloid-β Aggregates in Alzheimer’s Disease",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:42Z",
  "url": "https://research.draconitic.com/paper/de0at0d8wvw3k",
  "venue": "Molecules",
  "versions": []
}