{
  "abstract": "Hydrogen sulfide (H 2 S) is a redox-active gasotransmitter implicated in tumor progression and immune regulation. The enzyme 3-mercaptopyruvate sulfurtransferase (3-MST) is a key contributor to endogenous H 2 S and polysulfide production, but its role in tumor–immune interactions remains poorly defined. Here, we show that 3-MST is the most abundantly expressed H 2 S-synthesizing enzyme in human renal cell carcinoma cells (RCC) and that high 3-MST expression correlates with reduced patient survival. Pharmacological inhibition of 3-MST lowered intracellular H 2 S levels in Renca renal carcinoma cells, suppressed proliferation, induced apoptosis, disrupted cellular metabolism, and increased expression of immune-related genes and proteins. In immune cells, partial inhibition of 3-MST promoted T cell activation, as evidenced by increased CD69 expression on CD3 + , CD4 + , and CD8 + T cells. In contrast, complete inhibition of 3-MST, achieved by high concentrations of the inhibitor, modestly reduced CD8 + T cell proliferation. Functionally, 3-MST inhibition potentiated antigen-specific CD8 + T cell-mediated killing of tumor cells, an effect further amplified by PD-L1 blockade. These results establish 3-MST as a redox-sensitive metabolic driver of tumor growth and immune evasion in RCC and demonstrate that its inhibition can boost antitumor immune responses, offering a potential avenue for combination immunotherapy.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Switzerland",
    "Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Switzerland; Institute of Pharmacology, University of Bern, Switzerland",
    "Institute of Pharmacology, University of Bern, Switzerland",
    "Section of Pharmacology, Department of Oncology, Microbiology and Immunology, University of Fribourg, Switzerland",
    "Theodor Kocher Institute, University of Bern, Switzerland",
    "Biochemistry Department, Faculty of Pharmacy and Biotechnology, German University in Cairo (GUC), Cairo, Egypt",
    "Pharmacology and Toxicology Department, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, Egypt; University of London at European Universities in Egypt (EUE), New Administrative Capital, Egypt",
    "Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Switzerland; Institute of Pharmacology, University of Bern, Switzerland; Department of Anaesthesiology, Pharmacology, Intensive Care and Emergency Medicine, University of Geneva, Switzerland; Corresponding author. Institute of Pharmacology, University of Bern, Switzerland."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Muriel Urwyler"
    },
    {
      "affs": [
        1
      ],
      "name": "Marietta Margareta Korsos"
    },
    {
      "affs": [
        1
      ],
      "name": "Eloise Dupuychaffray"
    },
    {
      "affs": [
        2
      ],
      "name": "Nikita Markov"
    },
    {
      "affs": [
        3
      ],
      "name": "Kelly Ascenção"
    },
    {
      "affs": [
        3
      ],
      "name": "Maria Petrosino"
    },
    {
      "affs": [
        3
      ],
      "name": "Karim Zuhra"
    },
    {
      "affs": [
        2
      ],
      "name": "Darko Stojkov"
    },
    {
      "affs": [
        4
      ],
      "name": "Sara Barcos"
    },
    {
      "affs": [
        0
      ],
      "name": "Jérémie Tachet"
    },
    {
      "affs": [
        0
      ],
      "name": "Pragallabh Purwar"
    },
    {
      "affs": [
        0
      ],
      "name": "Montserrat Alvarez"
    },
    {
      "affs": [
        0
      ],
      "name": "Aurélien Pommier"
    },
    {
      "affs": [
        5
      ],
      "name": "Mohamed Z. Gad"
    },
    {
      "affs": [
        6
      ],
      "name": "Reham M. Abdel-Kader"
    },
    {
      "affs": [
        3
      ],
      "name": "Csaba Szabo"
    },
    {
      "affs": [
        7
      ],
      "name": "Carole Bourquin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/de0zsxgf6pqfr.bib",
  "cited_by": 0,
  "doi": "10.1016/j.redox.2026.104373",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2213231726003721",
  "id": "de0zsxgf6pqfr",
  "issns": [
    "2213-2317"
  ],
  "landing_url": "https://doi.org/10.1016/j.redox.2026.104373",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ],
    [
      "lcc",
      "R5-920"
    ],
    [
      "openalex",
      "1303|Sulfur Compounds in Biology"
    ]
  ],
  "openalex": "W4414430988",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S2213231726003721/pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/de0zsxgf6pqfr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:43:15Z",
      "native_id": "6bcfa98a13074411af3dc4969219f280",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.redox.2026.104373"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "journal-openalex:1312|Redox biology and oxidative stress",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "lcc:QH301-705.5",
      "subfield": "general"
    },
    {
      "field": "medicine",
      "origin": "lcc:R5-920",
      "subfield": "general"
    }
  ],
  "title": "Inhibition of 3-mercaptopyruvate sulfurtransferase enhances CD8",
  "title_text": "Inhibition of 3-mercaptopyruvate sulfurtransferase enhances CD8",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:43:15Z",
  "url": "https://research.draconitic.com/paper/de0zsxgf6pqfr",
  "venue": "Redox Biology",
  "versions": []
}