{
  "abstract": "3′-O-amino-deoxyribonucleoside triphosphates (3′-ONH2-dNTPs) are crucial reversible terminators for template-independent DNA synthesis. Owing to the structural complexity of purine bases, here we propose a chemo-enzymatic process for the synthesis of purine-type 3′-ONH2-dNTPs. First, the aminoalkoxyl group was introduced into commercially available dA and dG via a chemical catalytic process, affording yields of 24.9% and 27.3%, respectively. Subsequently, to establish a green and scalable enzymatic cascade for phosphorylation of the substrates, we engineered two key phosphorylation enzymes (Dm-dNK and MrPPK2), improving the rate-limiting monophosphorylation of 3′-ONH2-dG from 26% to 97.8% and achieving 59.9% conversion to the corresponding triphosphate. Lastly, the produced nucleotides exhibit performance equivalent to commercial counterparts in terminal deoxynucleotidyl transferase (TdT)-mediated DNA synthesis, supporting the construction of 120-nt strands with ∼99.7% stepwise accuracy. This work establishes an efficient and generalizable catalytic platform for producing modified dNTPs, advancing the practical application of enzymatic DNA synthesis technologies.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China",
    "Zhonghe Gene Technology Co., Ltd., Tianjin 300308, China",
    "Haihe Laboratory of Synthetic Biology, Tianjin 300308, China",
    "College of Life Sciences, Nankai University, Tianjin 300071, P. R. China",
    "Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China",
    "College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Qin He"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Shuai Cheng"
    },
    {
      "affs": [
        1
      ],
      "name": "Jianqing Yue"
    },
    {
      "affs": [
        3
      ],
      "name": "Shu Zong"
    },
    {
      "affs": [
        1
      ],
      "name": "Congyu Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Xuerong Qi"
    },
    {
      "affs": [
        1
      ],
      "name": "Lipei Liu"
    },
    {
      "affs": [
        4
      ],
      "name": "Zhidan Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Jun Sun"
    },
    {
      "affs": [
        5
      ],
      "name": "Jian Cheng"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaoyun Lu"
    },
    {
      "affs": [
        5
      ],
      "name": "Huifeng Jiang",
      "orcid": "0000-0002-3757-7896"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/de9e0pm7ys368.bib",
  "cited_by": 0,
  "doi": "10.1021/cbe.6c00032",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13697939/",
  "id": "de9e0pm7ys368",
  "issns": [
    "2836-967X"
  ],
  "landing_url": "https://doi.org/10.1021/cbe.6c00032",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1312|DNA and Nucleic Acid Chemistry"
    ]
  ],
  "openalex": "W7202030632",
  "pdf_url": "https://europepmc.org/articles/PMC13697939?pdf=render",
  "pmcid": "PMC13697939",
  "pmid": "42936496",
  "published": "2026-08-09",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/de9e0pm7ys368.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:54Z",
      "native_id": "PMC13697939",
      "source": "pmc",
      "url": "https://doi.org/10.1021/cbe.6c00032"
    },
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42936496",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42936496"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|DNA and Nucleic Acid Chemistry",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Chemo-Enzymatic Synthesis of Purine 3′-O-Amino-Deoxyribonucleoside Triphosphates",
  "title_text": "Chemo-Enzymatic Synthesis of Purine 3′-O-Amino-Deoxyribonucleoside Triphosphates",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:53Z",
  "url": "https://research.draconitic.com/paper/de9e0pm7ys368",
  "venue": "Chem & Bio Engineering",
  "versions": []
}