{
  "abstract": "Understanding biological systems requires observing features and processes across vast spatial and temporal scales, spanning nanometers to centimeters and milliseconds to days, often using multiple imaging modalities within complex native microenvironments. Yet, achieving this comprehensive view is challenging because microscopes optimized for specific tasks typically lack versatility due to inherent optical and sample handling trade-offs, and frequently suffer performance degradation from sample-induced optical aberrations in multicellular contexts. Here, we present MOSAIC, a reconfigurable microscope that integrates multiple advanced imaging techniques including light-sheet, label-free, super-resolution, and multi-photon, all equipped with adaptive optics. MOSAIC enables non-invasive imaging of subcellular dynamics in both cultured cells and live multicellular organisms, nanoscale mapping of molecular architectures across millimeter-scale expanded tissues, and structural/functional neural imaging within live mice. MOSAIC facilitates correlative studies across biological scales within the same specimen, providing an integrated platform for broad biological investigation.",
  "abstract_shown": true,
  "affiliations": [
    "Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA",
    "Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 2047, USA",
    "Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA",
    "Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 9470, USA",
    "Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA",
    "Department of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA",
    "Chicago, IL 60607, USA",
    "Department of Chemistry, University of Illinois Chicago",
    "Monash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, VIC, 3800, Australia",
    "Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA",
    "Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA",
    "Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA",
    "Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA, USA",
    "Department of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine 92697, USA",
    "Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States",
    "Department of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA",
    "Neuroimaging Laboratory, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN, 38105, USA",
    "McKusick-Nathans Institute and the Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA",
    "Wilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA",
    "University of Toronto, Department of Cell and Systems Biology, Scarborough, Ontario, Canada",
    "University of Washington BioRepository and Integrated Neuropathology (BRaIN) lab, Harborview Medical Center, Seattle, WA, USA",
    "Department of Cell Biology, Harvard Medical School, Boston, MA 02115",
    "Department of Pediatrics, Harvard Medical School, Boston, MA 02115",
    "Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115",
    "Department of Biomedical Engineering, and Center for Complex Systems Biology, University of California, Irvine, CA 92697 University of California, Irvine, Irvine 92697, USA",
    "European Molecular Laboratory Australia, Monash University, Clayton/ Melbourne, VIC 3800, Australia",
    "Department of Biological Sciences, University of Illinois Chicago, Chicago, IL 60607, USA",
    "Department of Physics, Howard Hughes Medical Institute, Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA",
    "Chan Zuckerberg Biohub, San Francisco, CA 94158, USA",
    "Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 50,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Tian-ming Fu B",
      "orcid": "0000-0001-6265-0859"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Gaoxiang Liu",
      "orcid": "0000-0002-1001-0064"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Daniel Edward Milkie",
      "orcid": "0000-0002-3917-6965"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Xiongtao Ruan",
      "orcid": "0000-0001-5322-3249"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Frederik Görlitz",
      "orcid": "0000-0002-8415-1820"
    },
    {
      "affs": [
        4
      ],
      "name": "Yu Shi",
      "orcid": "0000-0002-8827-7500"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Valentina Ferro",
      "orcid": "0000-0002-9891-6468"
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    {
      "affs": [
        2,
        3
      ],
      "name": "Nikita S. Divekar",
      "orcid": "0009-0006-9384-8407"
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    {
      "affs": [
        5,
        6,
        7
      ],
      "name": "Wei Wang",
      "orcid": "0000-0001-5508-7359"
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    {
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        8
      ],
      "name": "Harrison M. York",
      "orcid": "0000-0002-4534-8188"
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      "name": "Velat Kilic",
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        0,
        3,
        1
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      "name": "Matthew Mueller",
      "orcid": "0000-0003-0774-1031"
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        9
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      "name": "Yajie Liang",
      "orcid": "0000-0002-4798-4882"
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        10
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      "name": "Timothy A. Daugird",
      "orcid": "0000-0002-6313-0246"
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        11
      ],
      "name": "María José Gacha Garay",
      "orcid": "0000-0002-9312-5237"
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        11
      ],
      "name": "Kathryn A. Larkin",
      "orcid": "0000-0003-2784-8290"
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        11
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      "name": "Rebecca C. Adikes",
      "orcid": "0000-0002-7526-8701"
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        3
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      "name": "N Harrison"
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        12,
        2,
        3
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      "name": "Cyna R. Shirazinejad",
      "orcid": "0000-0003-1006-5775"
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        2,
        3
      ],
      "name": "Shara C. Williams"
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      "affs": [
        13
      ],
      "name": "Jamison L. Nourse",
      "orcid": "0000-0001-6240-3209"
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        0,
        1
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      "name": "Shu‐Hsien Sheu",
      "orcid": "0000-0003-0758-4654"
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    {
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        0,
        1
      ],
      "name": "Liang Gao",
      "orcid": "0000-0001-9983-0626"
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      "affs": [
        14
      ],
      "name": "Tongchao Li",
      "orcid": "0000-0002-2113-6359"
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    {
      "affs": [
        15
      ],
      "name": "Chandrani Mondal"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Kemal Achour"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Wilmene Hercule"
    },
    {
      "affs": [
        16
      ],
      "name": "Daniel R. Stabley",
      "orcid": "0000-0001-9187-6099"
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    {
      "affs": [
        17,
        18
      ],
      "name": "Kevin Emmerich",
      "orcid": "0000-0002-0088-1333"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Peng Dong",
      "orcid": "0000-0003-4946-1409"
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    {
      "affs": [
        2,
        3
      ],
      "name": "David G. Drubin",
      "orcid": "0000-0003-3002-6271"
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    {
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        0,
        1
      ],
      "name": "Zhe J. Liu",
      "orcid": "0000-0002-3592-3150"
    },
    {
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        0,
        1
      ],
      "name": "David E. Clapham",
      "orcid": "0000-0002-4459-9428"
    },
    {
      "affs": [
        17,
        18
      ],
      "name": "Jeff S. Mumm",
      "orcid": "0000-0002-2575-287X"
    },
    {
      "affs": [
        19
      ],
      "name": "Minoru Koyama",
      "orcid": "0000-0002-9774-9223"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Alison N. Killilea",
      "orcid": "0000-0002-8308-8196"
    },
    {
      "affs": [
        15
      ],
      "name": "Jose Javier Bravo‐Cordero",
      "orcid": "0000-0001-9481-1231"
    },
    {
      "affs": [
        20
      ],
      "name": "Christopher Dirk Keene",
      "orcid": "0000-0002-5291-1469"
    },
    {
      "affs": [
        14
      ],
      "name": "Liqun Luo",
      "orcid": "0000-0001-5467-9264"
    },
    {
      "affs": [
        21,
        22,
        23
      ],
      "name": "Tomas L. Kirchhausen",
      "orcid": "0000-0003-0559-893X"
    },
    {
      "affs": [
        24,
        13
      ],
      "name": "Medha M. Pathak",
      "orcid": "0000-0002-6518-3085"
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    {
      "affs": [
        25,
        8
      ],
      "name": "Senthil Arumugam",
      "orcid": "0000-0001-6733-4679"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "James K. Nuñez",
      "orcid": "0000-0002-7396-6119"
    },
    {
      "affs": [
        26,
        5,
        6
      ],
      "name": "Ruixuan Gao",
      "orcid": "0000-0001-7137-2512"
    },
    {
      "affs": [
        11
      ],
      "name": "David Q. Matus",
      "orcid": "0000-0002-1570-5025"
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    {
      "affs": [
        11
      ],
      "name": "Benjamin L. Martin",
      "orcid": "0000-0001-5474-4492"
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    {
      "affs": [
        2,
        3
      ],
      "name": "Ian A. Swinburne",
      "orcid": "0000-0003-4162-0508"
    },
    {
      "affs": [
        2,
        27,
        0,
        3,
        1
      ],
      "name": "Eric Betzig",
      "orcid": "0000-0002-4192-1274"
    },
    {
      "affs": [
        10,
        4
      ],
      "name": "Wesley R. Legant",
      "orcid": "0000-0003-1827-5371"
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      "affs": [
        28,
        2,
        29,
        3
      ],
      "name": "Srigokul Upadhyayula",
      "orcid": "0000-0001-6727-5883"
    }
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  "bibtex": "https://research.draconitic.com/paper/desqs54h103nx.bib",
  "cited_by": 11,
  "doi": "10.1038/s41592-026-03066-1",
  "fulltext_url": null,
  "id": "desqs54h103nx",
  "issns": [
    "1548-7091",
    "1548-7105"
  ],
  "landing_url": "https://doi.org/10.1038/s41592-026-03066-1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "1304|Advanced Fluorescence Microscopy Techniques"
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  "pdf_url": null,
  "pmcid": "PMC12259214",
  "pmid": "40661351",
  "published": "2026-05-22",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/desqs54h103nx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:48Z",
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      "url": "https://doi.org/10.1038/s41592-026-03066-1"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
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  ],
  "title": "A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms",
  "title_text": "A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T13:20:43Z",
  "url": "https://research.draconitic.com/paper/desqs54h103nx",
  "venue": "Nature Methods",
  "versions": []
}