{
  "abstract": "IntroductionPeanut southern blight, caused by Sclerotium rolfsii, is a devastating soilborne disease that threatens peanut production worldwide. Members of Burkholderia have attracted attention as biocontrol agents owing to their diverse secondary metabolites and plant growth-promoting (PGP) capacity.MethodsWe isolated B. gladioli PNB20-1 from the disease-healthy junction of peanut plants. Its biocontrol and PGP potential was evaluated by dual-culture, greenhouse pot, and biosafety assays, its genome was sequenced on the Oxford Nanopore platform, and host defense responses were profiled by RNA-Seq.ResultsPNB20-1 exhibited in vitro antagonism against S. rolfsii with a 52.7% inhibition rate and reduced sclerotial biomass by 19.3% after 4 weeks of co-culture. The complete genome of PNB20-1 comprised 8,278,972 bp across three circular replicons, encoding 6,923 CDSs and 21 biosynthetic gene clusters (BGCs). Among these, the ornibactin-type siderophore BGC and phosphate solubilization genes were supported by phenotypic assays. Transcriptomic profiling of PNB20-1-inoculated peanut roots identified 1,552 differentially expressed genes, of which 1,057 were upregulated and 495 downregulated. GO and KEGG enrichment analyses pointed to a coordinated defense response encompassing stilbenoid phytoalexin biosynthesis, cutin-, suberin-, and lignin-mediated apoplastic barrier reinforcement, and salicylic acid (SA)-dominated multi-hormonal signaling. Preliminary biosafety evaluation showed that PNB20-1 is non-hemolytic and lacks key virulence determinants of clinically significant Burkholderia species.DiscussionThese findings suggest that B. gladioli PNB20-1 is a promising biocontrol agent and provide insights into the molecular basis of Burkholderia-mediated disease suppression in peanut.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Original Research",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Jinyan Xu"
    },
    {
      "name": "Yonghui Liu"
    },
    {
      "name": "Man Liang"
    },
    {
      "name": "Weiqi Chen"
    },
    {
      "name": "Yi Shen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dg61z4y932tgk.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1984673",
  "fulltext_url": null,
  "id": "dg61z4y932tgk",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1984673",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "biology/microbiology"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-04",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/dg61z4y932tgk.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:20:46Z",
      "native_id": "10.3389/fmicb.2026.1984673",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1984673"
    },
    {
      "first_seen": "2026-11-04T12:21:25Z",
      "native_id": "10.3389/fmicb.2026.1984673",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1984673/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "feed-journal:biology/microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Integrated genomic and transcriptomic analysis reveals the biocontrol and plant growth-promoting mechanisms of Burkholderia gladioli PNB20-1 against peanut southern blight",
  "title_text": "Integrated genomic and transcriptomic analysis reveals the biocontrol and plant growth-promoting mechanisms of Burkholderia gladioli PNB20-1 against peanut southern blight",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T00:21:17Z",
  "url": "https://research.draconitic.com/paper/dg61z4y932tgk",
  "venue": "Frontiers in Microbiology",
  "versions": []
}