{
  "abstract": "Background\n\nIn a randomized, blinded, dual center study across two European primate research facilities, we evaluated the immunogenicity and protective efficacy of a DNA prime-oropharyngeal adenoviral boost vaccination strategy against respiratory syncytial virus (RSV).Methods\n\nRhesus macaques received an RSV fusion protein (F) DNA vaccine via electroporation, followed by two mucosal administrations of a recombinant adenovirus vector encoding the same antigen. Mock-vaccinated animals and a comparator group mimicking natural immunity induced by primary RSV infection were included as controls. Systemic and mucosal immune responses were monitored longitudinally.Results\n\nWe detected robust cellular and humoral immune responses, but with some variability between the two centers. Nevertheless, RSV-vaccinated animals demonstrated markedly reduced viral replication in the upper and lower respiratory tract after RSV challenge infection. Unexpected RSV-specific immunity in the control group at one single study site prevented confirmation of the predefined primary endpoint.Conclusion\n\nOur results support the potential of mucosal adenoviral boosting following DNA priming to induce protective immunity against RSV, while highlighting challenges associated with multi-center preclinical vaccine studies.",
  "abstract_shown": true,
  "affiliations": [
    "Harald zur Hausen Institute of Virology, Uniklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany.",
    "FAU Profile Center Immunomedicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.",
    "Biomedical Primate Research Centre, Rijswijk, the Netherlands.",
    "German Primate Center, Göttingen, Germany.",
    "Department of Medical Informatics, Biometry and Epidemiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Matthias Tenbusch",
      "orcid": "0000-0003-3951-9056"
    },
    {
      "affs": [
        2
      ],
      "name": "Gerrit Koopman",
      "orcid": "0000-0002-3428-5978"
    },
    {
      "affs": [
        2
      ],
      "name": "Petra Mooij",
      "orcid": "0000-0001-8608-8629"
    },
    {
      "affs": [
        3
      ],
      "name": "Berit Roshani",
      "orcid": "0000-0003-0109-9703"
    },
    {
      "affs": [
        0
      ],
      "name": "Pascal Irrgang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Dennis Lapuente"
    },
    {
      "affs": [
        2
      ],
      "name": "Ivanela Kondova",
      "orcid": "0000-0003-2745-6094"
    },
    {
      "affs": [
        2
      ],
      "name": "Willy M Bogers",
      "orcid": "0000-0002-4563-8513"
    },
    {
      "affs": [
        2
      ],
      "name": "Edmond J Remarque",
      "orcid": "0000-0002-5412-6958"
    },
    {
      "affs": [
        3
      ],
      "name": "Ramona Vestweber"
    },
    {
      "affs": [
        3
      ],
      "name": "Samuel Alberto Merida Ruiz"
    },
    {
      "affs": [
        3
      ],
      "name": "Nadine Krüger",
      "orcid": "0000-0002-4413-8699"
    },
    {
      "affs": [
        4
      ],
      "name": "Sebastian Meyer",
      "orcid": "0000-0002-1791-9449"
    },
    {
      "affs": [
        4
      ],
      "name": "Olaf Gefeller",
      "orcid": "0000-0002-8985-7582"
    },
    {
      "affs": [
        3
      ],
      "name": "Christiane Stahl-Hennig"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Klaus Überla",
      "orcid": "0000-0001-7822-3835"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dgbnbpn5kc7v3.bib",
  "cited_by": 0,
  "doi": "10.1111/jmp.70121",
  "fulltext_url": "https://europepmc.org/articles/PMC13682502",
  "id": "dgbnbpn5kc7v3",
  "issns": [
    "0047-2565",
    "1600-0684"
  ],
  "landing_url": "https://europepmc.org/article/MED/42899948",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Respiratory viral infections research"
    ]
  ],
  "openalex": "W7223068567",
  "pdf_url": "https://europepmc.org/articles/PMC13682502?pdf=render",
  "pmcid": "PMC13682502",
  "pmid": "42899948",
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dgbnbpn5kc7v3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:32Z",
      "native_id": "MED:42899948",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42899948"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Respiratory viral infections research",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "A Confirmatory, Dual Center Nonhuman Primate Study on the Efficacy of Novel Oropharyngeal Spray Immunization With an Adenoviral Vector Vaccine Against RSV-Important Lessons Learned.",
  "title_text": "A Confirmatory, Dual Center Nonhuman Primate Study on the Efficacy of Novel Oropharyngeal Spray Immunization With an Adenoviral Vector Vaccine Against RSV-Important Lessons Learned.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:32Z",
  "url": "https://research.draconitic.com/paper/dgbnbpn5kc7v3",
  "venue": "Journal of medical primatology",
  "versions": []
}