{
  "abstract": "Introduction\n\nIn bone tuberculosis, Mycobacterium tuberculosis (Mtb) infection triggers inflammatory bone destruction, but the macrophage programs that convert Mtb signals into osteoclastogenesis remain poorly defined.Methods\n\nWe generated single-cell RNA-seq data from human spinal tuberculosis lesions and integrated them with public H3K27ac ChIP-seq, RNA-seq, Hi-C, and motif data. Key findings were validated in primary macrophages stimulated with Mtb lysate.Results\n\nWe identified 64 Mtb-induced super-enhancers, and integrated analysis prioritized RIPK2 as a candidate. Lesion-associated myeloid populations showed enhanced inflammatory and osteoclast-priming signatures, followed a mononuclear phagocyte-to-osteoclast trajectory, and exhibited a linear correlation between RIPK2 expression and osteoclast score. In mice, RIPK2 localized to CTSK-positive bone interfaces. In vitro, Mtb stimulation induced Il1b, Nfkbiz, and Tnfrsf11a expression, IL-1β secretion, and surface RANK upregulation; these responses were suppressed by the RIPK2 inhibitor GSK583.Discussion\n\nThese findings link RIPK2 to both macrophage inflammatory signaling and osteoclastogenesis in bone tuberculosis, suggesting that RIPK2 may be a target for limiting infection-driven bone destruction.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Orthopedics, Southwest Hospital, Army Medical University, Chongqing, China.",
    "School of Medical and Information Engineering, Gannan Medical University, Ganzhou, Jiangxi, China.",
    "Department of Orthopaedics, 72nd Group Army Hospital, Huzhou Normal University, Huzhou, Zhejiang, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yuyang Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Ziyang Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Dongyang Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiulin Tan"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhaoxin Ma"
    },
    {
      "affs": [
        1
      ],
      "name": "Qingyang Xiao"
    },
    {
      "affs": [
        0
      ],
      "name": "Jie Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Qixiu Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Zehua Zhang"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Yong Tang"
    },
    {
      "affs": [
        0
      ],
      "name": "Ce Dou"
    },
    {
      "affs": [
        0
      ],
      "name": "Fei Luo"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dgya3gscm37yp.bib",
  "cited_by": 0,
  "doi": "10.3389/fcimb.2026.1970405",
  "fulltext_url": "https://europepmc.org/articles/PMC13686828",
  "id": "dgya3gscm37yp",
  "issns": [
    "2235-2988"
  ],
  "landing_url": "https://europepmc.org/article/MED/42922958",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2725|Tuberculosis Research and Epidemiology"
    ]
  ],
  "openalex": "W7220901249",
  "pdf_url": "https://europepmc.org/articles/PMC13686828?pdf=render",
  "pmcid": "PMC13686828",
  "pmid": "42922958",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dgya3gscm37yp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:33Z",
      "native_id": "MED:42922958",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42922958"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Tuberculosis Research and Epidemiology",
      "subfield": "infectious-disease"
    }
  ],
  "title": "A RIPK2-associated macrophage program links mycobacterial sensing to osteoclastogenesis in bone tuberculosis.",
  "title_text": "A RIPK2-associated macrophage program links mycobacterial sensing to osteoclastogenesis in bone tuberculosis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:33Z",
  "url": "https://research.draconitic.com/paper/dgya3gscm37yp",
  "venue": "Frontiers in cellular and infection microbiology",
  "versions": []
}