{
  "abstract": "High-resolution respirometry (HRR) of peripheral blood mononuclear cells (PBMCs) relies on controlled preanalytical procedures. Here, we present an optimized workflow for PBMC isolation from human whole blood, followed by short-term cryopreservation at -80°C. The protocol includes rapid thawing to preserve cell viability, washing steps to minimize platelet contamination, and quality-control assessment of PBMC number, viability, and composition before HRR measurements. Critical handling steps are optimized and validated by HRR against freshly prepared cells.",
  "abstract_shown": true,
  "affiliations": [
    "VASCage-Centre on Clinical Stroke Research, Innsbruck 6020, Austria. Electronic address: alejandra.romero-martinez@vascage.at.",
    "Oroboros Instruments, Innsbruck 6020, Austria.",
    "Oroboros Instruments, Innsbruck 6020, Austria. Electronic address: jaime.willis@oroboros.at.",
    "Department of Neurology, Medical University of Innsbruck, Innsbruck 6020, Austria.",
    "VASCage-Centre on Clinical Stroke Research, Innsbruck 6020, Austria."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Alejandra Romero",
      "orcid": "0000-0003-4759-3280"
    },
    {
      "affs": [
        1
      ],
      "name": "Alba Timón-Gómez"
    },
    {
      "affs": [
        2
      ],
      "name": "Jaime R Willis"
    },
    {
      "affs": [
        3
      ],
      "name": "Anja Perfler"
    },
    {
      "affs": [
        4
      ],
      "name": "Theresa Lindlbauer"
    },
    {
      "affs": [
        4
      ],
      "name": "Michael Knoflach"
    },
    {
      "affs": [
        1
      ],
      "name": "Erich Gnaiger"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dh1n2qsbnsam9.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104881",
  "fulltext_url": "https://europepmc.org/articles/PMC13676717",
  "id": "dh1n2qsbnsam9",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42842376",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Mitochondrial Function and Pathology"
    ]
  ],
  "openalex": "W7220832688",
  "pdf_url": "https://europepmc.org/articles/PMC13676717?pdf=render",
  "pmcid": "PMC13676717",
  "pmid": "42842376",
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dh1n2qsbnsam9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42842376",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42842376"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Mitochondrial Function and Pathology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for isolation and cryopreservation of human peripheral blood mononuclear cells for high-resolution respirometry.",
  "title_text": "Protocol for isolation and cryopreservation of human peripheral blood mononuclear cells for high-resolution respirometry.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/dh1n2qsbnsam9",
  "venue": "STAR protocols",
  "versions": []
}