{
  "abstract": "IntroductionMolecular screening for zoonotic and species-specific viral pathogens in environmental samples is essential for obtaining epidemiological data to support risk-based assessments in the food industry, particularly in settings such as slaughterhouses.MethodsThis study screened 72 effluent specimens collected between January and December 2025 from two sites within a domestic pig slaughterhouse in Central Italy: the stocking yard (V1) and the wastewater treatment plant (WWTP; V2). The viruses investigated were astrovirus, hepatitis A virus, hepatitis E virus (HEV), norovirus (NoV), and rotavirus (RV). Viral detection was performed using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) and nested reverse transcription polymerase chain reaction (RT-PCR) assays, followed by phylogenetic analysis.ResultsThe results showed that ribonucleic acid (RNA) from at least one of the target viruses was detected in 37.50% of the specimens, with HEV3-c detected in 26.39%, NoV-GII.3 in 12.50%, and NoV-GI.4 in 9.72%. Among the positive effluent samples, 62.96% were collected at V1 and 37.04% at V2. Samples from V1 exhibited higher viral loads, with HEV RNA levels reaching (105) genome equivalents per milliliter (GE/mL) and NoV-GI and NoV-GII RNA levels reaching (103) GE/mL, compared with (103) GE/mL for HEV RNA and (101) GE/mL for NoV-GI and NoV-GII RNA in samples from V2.DiscussionThe detection of viral RNA sequences both before and after WWTP treatment highlights scientific and regulatory concerns regarding the potential dissemination of zoonotic and species-specific viruses into the environment. Regulatory gaps in the management and monitoring of these environmental matrices underscore the importance of multidisciplinary approaches to addressing this One Health issue.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Original Research",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Gianluigi Ferri"
    },
    {
      "name": "Giovanni Loris Alborali"
    },
    {
      "name": "Alberto Olivastri"
    },
    {
      "name": "Maria Antonietta Piccioni"
    },
    {
      "name": "Adriana Ianieri"
    },
    {
      "name": "Alberto Vergara"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/djzeejfwg2qqe.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1957130",
  "fulltext_url": null,
  "id": "djzeejfwg2qqe",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1957130",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "biology/microbiology"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-29",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/djzeejfwg2qqe.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:18Z",
      "native_id": "10.3389/fmicb.2026.1957130",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1957130"
    },
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1957130",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1957130/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "feed-journal:biology/microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Viral pathogens in effluent samples collected from a swine slaughterhouse in Central Italy: a 1-year molecular surveillance",
  "title_text": "Viral pathogens in effluent samples collected from a swine slaughterhouse in Central Italy: a 1-year molecular surveillance",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T00:20:59Z",
  "url": "https://research.draconitic.com/paper/djzeejfwg2qqe",
  "venue": "Frontiers in Microbiology",
  "versions": []
}