{
  "abstract": "BackgroundCD177gp, also known as human neutrophil antigen (HNA)-2, is encoded by the CD177 gene located on chromosome 19. Different molecular mechanisms involving polymorphisms in the gene, mRNA splicing defects, DNA methylation, and so on govern the expression of HNA-2 on neutrophils ranging from 0% to 100%, with the unique biphenotypic expression observed in the majority of individuals. Its genotyping is often complicated by the presence of the pseudogene, CD177P1.MethodsIn the present study, the CD177gp expression on the neutrophils of 102 individuals from Mumbai, India, was compared via flow cytometry using two clones, namely MEM-166 and TAG4. For genotyping of the CD177 gene, long-range PCR was employed. Phenotypic expression was then correlated with the CD177 gene polymorphisms to determine the molecular basis of HNA-2 in Indians.ResultsOverall, the median CD177 expression using the MEM-166 clone was 60.4% (Q1–Q3: 48.5–70.8), and using the TAG4 clone, it was 58.4% (Q1–Q3: 45.9–72.9). A statistically significant difference of 1.56% clone-dependent variation in expression was observed between the two clones (p = 0.007). On molecular genotyping, 17 different genotype patterns and 8 novel mutations were identified. The multi-locus genotype pattern containing NM_020406.4:c.787A>T was found in a heterozygous state in 8.8% of the samples, of which 66.67% of the samples had low expression of CD177gp.ConclusionThis is the first study reporting the molecular characterization of the CD177 gene in individuals from Mumbai, India.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Original Research",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Harita Maru"
    },
    {
      "name": "Amrutha Jose"
    },
    {
      "name": "Manisha Madkaikar"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dmz5k277274ay.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1961339",
  "fulltext_url": null,
  "id": "dmz5k277274ay",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1961339",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "biology/immunology"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/dmz5k277274ay.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T06:20:04Z",
      "native_id": "10.3389/fimmu.2026.1961339",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1961339"
    },
    {
      "first_seen": "2026-11-06T12:21:13Z",
      "native_id": "10.3389/fimmu.2026.1961339",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1961339/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "feed-journal:biology/immunology",
      "subfield": "immunology"
    }
  ],
  "title": "CD177 gene polymorphisms governing the differential expression of HNA-2 on neutrophils in Indians",
  "title_text": "CD177 gene polymorphisms governing the differential expression of HNA-2 on neutrophils in Indians",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T00:22:43Z",
  "url": "https://research.draconitic.com/paper/dmz5k277274ay",
  "venue": "Frontiers in Immunology",
  "versions": []
}