{
  "abstract": "The rational construction of recyclable protein‐based catalytic materials requires connecting molecular enzyme design with higher‐order supramolecular organization and functional recovery interfaces. This study establishes a hierarchical design strategy that integrates fusion‐enzyme engineering, amyloid‐guided self‐assembly, and magnetic immobilization. The primary amine oxidase DPAO4 and the flavin‐containing amine oxidase DMAO7 were joined through flexible or rigid linkers, and the rigid (EAAAK)3 variant provided broad substrate coverage, greater tolerance to process‐relevant stresses, and lower interdomain fluctuations in molecular dynamics simulations. N‐terminal Sup351‐61 or Ure21‐80 domains promoted the formation of ThT‐responsive fibrillar assembly, and the resulting enzymatic nanofibers were coupled to amino‐functionalized Fe3O4@SiO2‐NH2 nanoparticles. The composites enabled magnetic separation within 30 s, catalyzed the degradation of eight biogenic amines under acidic, thermal, saline, and ethanol‐containing conditions, and retained an average of 53.2% of their initial activity after four recovery cycles. As proof‐of‐concept evaluation in complex matrices, selected systems removed up to 70.6% of total biogenic amines from fermented beverages and 69.7% from soy sauce. The distinct responses of the Sup35‐ and Ure2‐based composites suggest an association between assembly‐domain identity and catalytic performance in complex matrices. This work demonstrates a hierarchical design approach for recyclable protein‐based catalytic materials and sustainable biocatalysis.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Food Science and Technology School of Food Science and Technology Jiangnan University Wuxi Jiangsu China",
    "Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health Jiangnan University (Shaoxing) Industrial Technology Research Institute Shaoxing Zhejiang China",
    "National Engineering Research Center of Huangjiu Zhejiang Guyuelongshan Shaoxing Wine Co., Ltd Shaoxing Zhejiang China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Shuangping Liu",
      "orcid": "0000-0002-4578-2697"
    },
    {
      "affs": [
        0
      ],
      "name": "Qilin Yang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qian Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Tiantian Liu"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Xiao Han"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Jian Mao",
      "orcid": "0000-0002-3221-2492"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dnmektb228shy.bib",
  "cited_by": 0,
  "doi": "10.1002/advs.78579",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735435/",
  "id": "dnmektb228shy",
  "issns": [
    "2198-3844"
  ],
  "landing_url": "https://doi.org/10.1002/advs.78579",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1312|Enzyme Catalysis and Immobilization"
    ]
  ],
  "openalex": "W7225305928",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/advs.78579",
  "pmcid": "PMC13735435",
  "pmid": "42985828",
  "published": "2026-11-05",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/dnmektb228shy.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:21:07Z",
      "native_id": "PMC13735435",
      "source": "pmc",
      "url": "https://doi.org/10.1002/advs.78579"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Enzyme Catalysis and Immobilization",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Amyloid‐Guided Assembly of Magnetically Recyclable Enzymatic Nanofibers Toward Sustainable Biocatalysis",
  "title_text": "Amyloid‐Guided Assembly of Magnetically Recyclable Enzymatic Nanofibers Toward Sustainable Biocatalysis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:07Z",
  "url": "https://research.draconitic.com/paper/dnmektb228shy",
  "venue": "Advanced Science",
  "versions": []
}