{
  "abstract": "UV/ozone (UVO) treatment is a versatile, solvent-free approach for modifying polymer surfaces in biomedical applications. Although its effects on RGD-dependent cell adhesion have been extensively studied, the mechanisms underlying non-RGD-dependent interactions, specifically platelet adhesion, remain poorly understood. In this study, we investigated the adhesion and activation of human platelets on polystyrene (PS) and cyclo-olefin polymer (COP) surfaces modified with 172 nm vacuum ultraviolet (VUV) light-induced UVO treatment. A distinctive non-monotonic relationship between UVO irradiation time and platelet response was observed: short-term treatment (1-2 min) maximized platelet density, with a substrate-dependent increase in platelet activation that was most pronounced on PS, whereas prolonged treatment (>10 min) slightly reduced platelet density compared to short-term treatment. Surface analyses by X-ray photoelectron spectroscopy (XPS), water contact angle (WCA), and atomic force microscopy (AFM) showed monotonic increases in oxygen content, hydrophilicity, and nanoscale roughness, and were accompanied by a two-orders-of-magnitude decrease in Young's modulus (from approximately 1500 MPa to 10 MPa). Quartz Crystal Microbalance with Dissipation (QCM-D) measurements of fibrinogen (Fg) adsorption indicated that short-term treatment promotes a \"side-on\" orientation of Fg, maximizing the exposure of γ-chain binding sites relevant to platelet integrin recognition. In contrast, long-term treatment leads to a crowded interfacial environment, likely disrupting stable protein presentation. Furthermore, excessive surface softening may impair platelet mechanosensing. These findings provide mechanistic insight into time-dependent UVO surface modification and offer a rational framework for optimizing the hemocompatibility of polymer-based medical devices.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Materials Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo, Yokohama226-8502, Japan.",
    "Institute for Materials Chemistry and Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka819-0395, Japan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Riko Kaizu"
    },
    {
      "affs": [
        1
      ],
      "name": "Aki Yamamoto"
    },
    {
      "affs": [
        1
      ],
      "name": "Masaru Tanaka",
      "orcid": "0000-0002-1115-2080"
    },
    {
      "affs": [
        0
      ],
      "name": "Tomohiro Hayashi",
      "orcid": "0000-0002-4065-1807"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dnp1apek3ta4r.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.langmuir.6c02349",
  "fulltext_url": "https://europepmc.org/articles/PMC13629682",
  "id": "dnp1apek3ta4r",
  "issns": [
    "0743-7463",
    "1520-5827"
  ],
  "landing_url": "https://europepmc.org/article/MED/42916823",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2508|Polymer Surface Interaction Studies"
    ]
  ],
  "openalex": "W7213453858",
  "pdf_url": "https://europepmc.org/articles/PMC13629682?pdf=render",
  "pmcid": "PMC13629682",
  "pmid": "42916823",
  "published": "2026-09-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dnp1apek3ta4r.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:02Z",
      "native_id": "MED:42916823",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42916823"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2508|Polymer Surface Interaction Studies",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Time-Dependent UV/Ozone Treatment Modulates Platelet Adhesion on Polystyrene and Cyclo-Olefin Polymer through Fibrinogen Orientation.",
  "title_text": "Time-Dependent UV/Ozone Treatment Modulates Platelet Adhesion on Polystyrene and Cyclo-Olefin Polymer through Fibrinogen Orientation.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:02Z",
  "url": "https://research.draconitic.com/paper/dnp1apek3ta4r",
  "venue": "Langmuir : the ACS journal of surfaces and colloids",
  "versions": []
}