{
  "abstract": "Opioid use disorder (OUD) disrupts hypothalamic stress signaling, yet the molecular state of human paraventricular nucleus (PVN) corticotropin-releasing hormone (CRH) neurons remains poorly defined. Using single-nucleus RNA sequencing of 343,819 human hypothalamic nuclei, we applied complementary human HYPOMAP/MapMyCells and mouse PVN Atlas strategies that independently converged on the standardized Allen Brain Map subcluster Splat 410 843, providing cross-atlas validation of a rare CRH-enriched PVN population. Integrated GO, Reactome and KEGG analysis revealed OUD-associated enrichment of synaptic, junctional, cytoskeletal, receptor-signaling and ion transport programs, with relative enrichment of RNA processing, ER Golgi/vesicular, endolysosomal and glycan-related functions in controls. Targeted neuropeptide analysis further identified nominal increases in OXT, GHR, GAL and PRLR and decreases in VIP, AGRP, NPY, CRHBP and CALCR. Together, these findings define a reproducible human PVN CRH population and identify coordinated pathway and neuropeptide remodeling in OUD.",
  "abstract_shown": true,
  "affiliations": [
    "Louise A. Faillace, MD Department of Psychiatry and Behavioral Sciences, McGovern Medical School"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "L. R. Barrall"
    },
    {
      "name": "P. J. Urbanczyk"
    },
    {
      "name": "B. Kluwe-Schiavon"
    },
    {
      "name": "L. Stertz"
    },
    {
      "name": "S. Rankothgedera"
    },
    {
      "name": "M. Castillo"
    },
    {
      "name": "P. H. Gunaratne"
    },
    {
      "affs": [
        0
      ],
      "name": "C. Walss-Bass"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dpjhtppp195jr.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.09.749970",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.09.749970v1.full",
  "id": "dpjhtppp195jr",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.09.749970v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genomics"
    ],
    [
      "openalex",
      "2804|Neuropeptides and Animal Physiology"
    ]
  ],
  "openalex": "W7212299441",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.09.749970v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dpjhtppp195jr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:25Z",
      "native_id": "10.64898/2026.09.09.749970",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.09.749970v3"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:genomics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Atlas-Guided Cell-Specific Transcriptomics Identifies Pathway-Level Remodeling of Human Hypothalamic CRH Neurons in Opioid Use Disorder",
  "title_text": "Atlas-Guided Cell-Specific Transcriptomics Identifies Pathway-Level Remodeling of Human Hypothalamic CRH Neurons in Opioid Use Disorder",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:37Z",
  "url": "https://research.draconitic.com/paper/dpjhtppp195jr",
  "venue": "bioRxiv",
  "versions": []
}