{
  "abstract": "Background/Objectives: Quercetin is a dietary flavonoid with potential anticancer activity, but comparative effects in malignant and non-malignant lung-derived epithelial cells remain incompletely characterized. Methods: A549 and BEAS-2B cells were exposed to quercetin (1–250 µM) for 24, 48, and 72 h, and MTT-derived cellular metabolic activity was measured. Mean normalized concentration–response curves from three independent biological experiments (n = 3) were fitted with four-parameter logistic models, and Greenhouse–Geisser-corrected cell line × concentration × time interactions were tested. Apoptosis-related gene expression was assessed by qRT-PCR after exposure to 45 µM quercetin for 48 h; 45 µM was not a discrete concentration in the MTT series and was close to the directly tested 40 µM condition. Results: Apparent MTT-derived IC50 values were 58.28 µM (95% CI 44.18–74.81) for A549 and 121.22 µM (89.50–164.66) for BEAS-2B at 48 h, and 2.73 µM (2.31–3.67) and 1.49 µM (1.27–1.77), respectively, at 72 h. Cell line × concentration interactions were significant at all exposure times, as was the cell line × concentration × time interaction; transcriptional profiles differed descriptively between cell lines. Conclusions: The fitted IC50 ordering reversed between 48 and 72 h, so a stable cancer-selective response is not supported. MTT reflects metabolic activity rather than cell death, and transcript changes do not establish functional apoptosis.",
  "abstract_shown": true,
  "affiliations": [
    "Pharmacy Services Program, Vocational School of Health Services, Lokman Hekim University, 06510 Ankara, Türkiye",
    "Department of Medical Genetics, Faculty of Medicine, KTO Karatay University, 42020 Konya, Türkiye",
    "Department of Medical Microbiology, Faculty of Medicine, Erzincan Binali Yıldırım University, 24100 Erzincan, Türkiye"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Deniz Özkan Vardar",
      "orcid": "0000-0003-0976-9556"
    },
    {
      "affs": [
        1
      ],
      "name": "Tuğçe Duran"
    },
    {
      "affs": [
        2
      ],
      "name": "Merve Aydın",
      "orcid": "0000-0002-1522-6083"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dpnmxqfq0nct1.bib",
  "cited_by": 0,
  "doi": "10.3390/metabo16100740",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707059/?tool=EBI",
  "id": "dpnmxqfq0nct1",
  "issns": [
    "2218-1989"
  ],
  "landing_url": "https://www.mdpi.com/2218-1989/16/10/740",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2736|Flavonoids in Medical Research"
    ]
  ],
  "openalex": "W7215114831",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707059/pdf/?tool=EBI",
  "pmcid": "PMC13707059",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/dpnmxqfq0nct1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "10.3390/metabo16100740",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-1989/16/10/740"
    },
    {
      "first_seen": "2026-10-30T12:21:40Z",
      "native_id": "PMC:PMC13707059",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13707059"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Flavonoids in Medical Research",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Quercetin Differentially Modulates MTT-Derived Cellular Metabolic Activity and Apoptosis-Related Gene Expression in A549 and BEAS-2B Cells",
  "title_text": "Quercetin Differentially Modulates MTT-Derived Cellular Metabolic Activity and Apoptosis-Related Gene Expression in A549 and BEAS-2B Cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:40Z",
  "url": "https://research.draconitic.com/paper/dpnmxqfq0nct1",
  "venue": "Metabolites",
  "versions": []
}