{
  "abstract": "Background\n\nVaccination represents a promising strategy for preventing hydatidosis. We aimed to characterize the physicochemical properties of adenylate kinase 8 from Echinococcus granulosus (EgADK8) and identify potential B- and T-cell epitopes as vaccine candidates.Methods\n\nMultiple web-based servers were employed to predict immunogenic epitopes and evaluate the physicochemical, antigenic, structural, and subcellular characteristics of EgADK8.Results\n\nEgADK8 was predicted to be an extracellular protein with a molecular weight of 38.78 kDa. The protein showed no allergenic potential and exhibited high hydrophilicity, predicted thermal stability, and 36 potential post-translational modification sites. The secondary structure was mainly composed of alpha helices and random coils. The highest-quality three-dimensional model generated by the Robetta server achieved a confidence score of 0.79. Three independent prediction servers identified 16 linear and 9 conformational B-cell epitopes. Furthermore, two helper T lymphocyte (HTL) epitopes with predicted IFN-γ-inducing potential were identified after comprehensive screening. Additionally, five immunogenic human cytotoxic T lymphocyte (CTL) epitopes with predicted IFN-γ-inducing potential and three murine CTL epitopes were identified.Conclusion\n\nThese findings provide preliminary computational evidence supporting further investigation of EgADK8 as a potential component of a multiepitope vaccine against CE. The main limitation of this study was its reliance on computational analyses. Therefore, experimental validation using the whole protein and/or selected epitopes, either individually or in combination with other antigens, is required.",
  "abstract_shown": true,
  "affiliations": [
    "School of Paramedical Sciences, Shiraz University of Medical Sciences, Darab, Iran.",
    "Department of Paraclinic, Faculty of Veterinary Science, Kunduz University, Kunduz province, Afghanistan.",
    "Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.",
    "Faculty of Veterinary Medicine, Islamic Azad University Science and Research Branch, Tehran, Iran.",
    "D.V.M. Graduated, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.",
    "Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.",
    "Department of Basic Medical Sciences, Khoy University of Medical Sciences, Khoy, Iran.",
    "Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.",
    "Department of Basic Medical Sciences, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran.",
    "Faculty of Veterinary Medicine, Semnan University, Semnan, Iran."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Bahareh Bahadori Mazidi"
    },
    {
      "affs": [
        1
      ],
      "name": "Zabihullah Fasehi"
    },
    {
      "affs": [
        2
      ],
      "name": "Hamed Abdollahi"
    },
    {
      "affs": [
        3
      ],
      "name": "Mehrnoosh Naderizadeh"
    },
    {
      "affs": [
        4
      ],
      "name": "Alireza Najafi"
    },
    {
      "affs": [
        5
      ],
      "name": "Shadan Ghiabi"
    },
    {
      "affs": [
        6
      ],
      "name": "Ali Asghari"
    },
    {
      "affs": [
        7
      ],
      "name": "Mohammad Fathollahzadeh"
    },
    {
      "affs": [
        8
      ],
      "name": "Hamidreza Majidiani"
    },
    {
      "affs": [
        9
      ],
      "name": "Leyli Shabani"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dpqmck2b850jb.bib",
  "cited_by": 0,
  "doi": "10.18502/ijpa.v21i3.22590",
  "fulltext_url": "https://europepmc.org/articles/PMC13726624",
  "id": "dpqmck2b850jb",
  "issns": [
    "1735-7020",
    "2008-238X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42974470",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "1312|vaccines and immunoinformatics approaches"
    ]
  ],
  "openalex": "W7212405880",
  "pdf_url": "https://europepmc.org/articles/PMC13726624?pdf=render",
  "pmcid": "PMC13726624",
  "pmid": "42974470",
  "published": "2026-07-01",
  "publisher": "Tehran University of Medical Sciences",
  "ris": "https://research.draconitic.com/paper/dpqmck2b850jb.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:49Z",
      "native_id": "MED:42974470",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42974470"
    },
    {
      "first_seen": "2026-11-05T12:22:55Z",
      "native_id": "PMC13726624",
      "source": "pmc",
      "url": "https://doi.org/10.18502/ijpa.v21i3.22590"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|vaccines and immunoinformatics approaches",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Exploring Adenylate Kinase 8 (ADK8) of Echinococcus granulosus as a Novel Immunogen against Cystic Echinococcosis: A Computational Perspective.",
  "title_text": "Exploring Adenylate Kinase 8 (ADK8) of Echinococcus granulosus as a Novel Immunogen against Cystic Echinococcosis: A Computational Perspective.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:22:31Z",
  "url": "https://research.draconitic.com/paper/dpqmck2b850jb",
  "venue": "Iranian journal of parasitology",
  "versions": []
}