{
  "abstract": "Neurons interact in networks distributed throughout the brain. While much effort has focused on whole-brain calcium imaging, advances in genetically encoded voltage indicators raise the question of whether it might be possible to image neuronal voltage across entire brains. Achieving this requires a microscope with high volumetric imaging rates and signal-to-noise ratio. Here we present a remote-scanning light-sheet microscope capable of imaging genetically encoded voltage indicator-expressing neurons distributed throughout much of the brain of larval zebrafish at a volumetric rate of 200.8 Hz. We measured voltage traces from approximately one-quarter of all brain neurons. We found that neurons firing at different times during a sequence occupied different locations: visually evoked sequences mapped across the optic tectum, whereas stimulus-independent bursts were mapped across the cerebellum and medulla. Imaging voltage of neurons distributed in many brain regions may open new frontiers for understanding fundamental neural system operations.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Media Arts and Sciences, MIT, Cambridge, MA, USA",
    "McGovern Institute for Brain Research, MIT, Cambridge, MA, USA",
    "Department of Brain and Cognitive Sciences, MIT, Cambridge, MA, USA",
    "Picower Institute for Learning and Memory, MIT, Cambridge, MA, USA",
    "Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA",
    "David H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA",
    "Center for Neurobiological Engineering, Yang Tan Collective, and K. Lisa Yang Center for Bionics at MIT, Cambridge, MA, USA. edboyden@mit.edu",
    "David H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA. edboyden@mit.edu",
    "Department of Biological Engineering, MIT, Cambridge, MA, USA. edboyden@mit.edu",
    "Department of Brain and Cognitive Sciences, MIT, Cambridge, MA, USA. edboyden@mit.edu",
    "Department of Media Arts and Sciences, MIT, Cambridge, MA, USA. edboyden@mit.edu",
    "McGovern Institute for Brain Research, MIT, Cambridge, MA, USA. edboyden@mit.edu",
    "Mindspan Institute, Cambridge, MA, USA. edboyden@mit.edu"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zeguan Wang",
      "orcid": "0000-0001-9149-3389"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Jie J. Zhang",
      "orcid": "0000-0002-9089-1462"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Panagiotis Symvoulidis",
      "orcid": "0000-0002-1396-3426"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Wei Guo",
      "orcid": "0000-0003-2150-7073"
    },
    {
      "affs": [
        4,
        1
      ],
      "name": "Davy Deng",
      "orcid": "0000-0001-5310-5883"
    },
    {
      "affs": [
        5,
        1
      ],
      "name": "Adam Amsterdam",
      "orcid": "0000-0002-6806-1518"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Lige Zhang",
      "orcid": "0009-0009-4639-8046"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Takato Honda",
      "orcid": "0000-0002-3327-8282"
    },
    {
      "affs": [
        2
      ],
      "name": "Steven Roche",
      "orcid": "0009-0001-0374-852X"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Matthew A. Wilson",
      "orcid": "0000-0001-7149-3584"
    },
    {
      "affs": [
        6,
        7,
        8,
        9,
        10,
        11,
        12
      ],
      "name": "Edward S. Boyden",
      "orcid": "0000-0002-0419-3351"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dqzs6x4w3af4c.bib",
  "cited_by": 18,
  "doi": "10.1038/s41592-026-03179-7",
  "fulltext_url": null,
  "id": "dqzs6x4w3af4c",
  "issns": [
    "1548-7091",
    "1548-7105"
  ],
  "landing_url": "https://doi.org/10.1038/s41592-026-03179-7",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
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  "openalex": "W7203498224",
  "pdf_url": null,
  "pmcid": "PMC10760087",
  "pmid": "38168290",
  "published": "2026-08-14",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/dqzs6x4w3af4c.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T13:20:46Z",
      "native_id": "W7203498224",
      "source": "openalex",
      "url": "https://doi.org/10.1038/s41592-026-03179-7"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Voltage imaging of neurons distributed across entire brains of larval zebrafish",
  "title_text": "Voltage imaging of neurons distributed across entire brains of larval zebrafish",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:05Z",
  "url": "https://research.draconitic.com/paper/dqzs6x4w3af4c",
  "venue": "Nature Methods",
  "versions": []
}