{
  "abstract": "Accurate measurement of enteric methane (CH₄) emissions is essential for methane phenotyping, but established techniques may be costly and difficult to apply to large numbers of animals. This study evaluated a simple mask-based system for measuring CH₄ concentration in exhaled breath of lactating dairy cows in comparison with CH₄ production measured using GreenFeed. Thirty-two Holstein cows were evaluated, generating 60 paired observations. On each sampling day, exhaled breath was collected using the mask during three 5 min periods, and CH₄ concentration was determined by micro gas chromatography. Mask CH₄ concentration was positively associated with GreenFeed CH₄ production (Pearson r = 0.616; Spearman ρ = 0.606; p < 0.001). The association remained significant after accounting for repeated observations within cows using a linear mixed-effects model (β = 53.1 ± 15.7 g/day per 0.1 percentage-point increase in mask CH₄, 95% CI: 22.3 to 84.0; p < 0.001). An exploratory within-cow analysis was also performed in four cows evaluated over five sampling days. Positive within-cow correlations were observed in all four animals (r = 0.157-0.699), but none reached statistical significance (p > 0.05). These results indicate that CH₄ concentration measured using the mask contains information on differences in methane emission among cows and may have potential as a methane phenotyping measure.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Animal Science, Food and Nutrition (DIANA), Faculty of Agricultural, Food and Environmental Sciences, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy.",
    "Department of Veterinary Sciences, Università di Messina, 98168 Messina, Italy.",
    "Romeo and Enrica Invernizzi Research Center for Sustainable Dairy Production of the Università Cattolica del Sacro Cuore (CREI), 29122 Piacenza, Italy."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Marta Sfulcini",
      "orcid": "0000-0002-3689-1883"
    },
    {
      "affs": [
        1
      ],
      "name": "Vincenzo Lopreiato",
      "orcid": "0000-0001-6965-7340"
    },
    {
      "affs": [
        0
      ],
      "name": "Fiorenzo Piccioli-Cappelli",
      "orcid": "0000-0003-1277-7821"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Erminio Trevisi",
      "orcid": "0000-0003-1644-1911"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrea Minuti",
      "orcid": "0000-0002-0617-6571"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dre1faffcsaks.bib",
  "cited_by": 0,
  "doi": "10.3390/ani16193156",
  "fulltext_url": "https://europepmc.org/articles/PMC13663346",
  "id": "dre1faffcsaks",
  "issns": [
    "2076-2615"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883125",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1102|Ruminant Nutrition and Digestive Physiology"
    ]
  ],
  "openalex": "W7220977380",
  "pdf_url": "https://europepmc.org/articles/PMC13663346?pdf=render",
  "pmcid": "PMC13663346",
  "pmid": "42883125",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dre1faffcsaks.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42883125",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883125"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Ruminant Nutrition and Digestive Physiology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Association Between Methane Concentration Measured by a Modified Mask System and GreenFeed Methane Production in Dairy Cows.",
  "title_text": "Association Between Methane Concentration Measured by a Modified Mask System and GreenFeed Methane Production in Dairy Cows.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/dre1faffcsaks",
  "venue": "Animals : an open access journal from MDPI",
  "versions": []
}