{
  "abstract": "Although the AP-1 clathrin adaptor has been studied for decades, its role in membrane trafficking has been ambiguous. Here, we systematically investigated the localization and function of mammalian AP-1. This adaptor associates with the trans-Golgi network (TGN) and with TGN-related recycling endosomes. Disruption of the 1A subunit of AP-1 has only a mild effect on the Golgi export of secretory cargoes, but it causes depletion from the Golgi of multiple transmembrane TGN proteins. Live-cell imaging and a cell surface labeling assay indicate that when TGN proteins are unable to bind AP-1, they exit the Golgi to the plasma membrane. Our interpretation is that the vesicles generated with AP-1 recycle transmembrane TGN proteins, thereby keeping those proteins in the Golgi during cisternal maturation. The clathrin adaptor epsinR seems to function as a partner for AP-1 in recycling a subset of TGN proteins. These results closely align with studies of AP-1 function in yeast, suggesting that the conserved core role of AP-1 is the intra-Golgi recycling of TGN proteins.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Fernando M. Valbuena"
    },
    {
      "name": "Maddison Lessard"
    },
    {
      "name": "Adam H. Krahn"
    },
    {
      "name": "Areti Pantazopoulou"
    },
    {
      "name": "Conor F. Lee-Smith"
    },
    {
      "name": "J. Christopher Fromme",
      "orcid": "0000-0002-8837-0473"
    },
    {
      "name": "Petia Adarska"
    },
    {
      "name": "Francesca Bottanelli"
    },
    {
      "name": "Benjamin S. Glick",
      "orcid": "0000-0002-7921-1374"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dsv6r0527wqwb.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.21.760840",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760840v1.full",
  "id": "dsv6r0527wqwb",
  "issns": [
    "2692-8205"
  ],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760840v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1307|Cellular transport and secretion"
    ]
  ],
  "openalex": "W7223170640",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760840v1.full.pdf",
  "pmcid": "PMC13738746",
  "pmid": "42996028",
  "published": "2026-10-21",
  "publisher": "Cold Spring Harbor Laboratory Preprints",
  "ris": "https://research.draconitic.com/paper/dsv6r0527wqwb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:09Z",
      "native_id": "10.64898/2026.10.21.760840",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760840v2"
    },
    {
      "first_seen": "2026-11-09T12:23:41Z",
      "native_id": "PMC13738746",
      "source": "pmc",
      "url": "https://doi.org/10.64898/2026.10.21.760840"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "The AP-1 clathrin adaptor has a conserved role in recycling resident proteins of the trans-Golgi network",
  "title_text": "The AP-1 clathrin adaptor has a conserved role in recycling resident proteins of the trans-Golgi network",
  "type": "preprint",
  "updated_at": "2026-11-09T12:23:41Z",
  "url": "https://research.draconitic.com/paper/dsv6r0527wqwb",
  "venue": "bioRxiv",
  "versions": []
}