{
  "abstract": "Here, we present a protocol to identify DNA methylation-sensitive enhancer sites using the leptin-regulating enhancer RS1 in adipose tissue. We describe steps for validating RS1’s endogenous function with a dCas9-KRAB repressor system and quantifying RS1 CpG methylation by pyrosequencing. We then detail procedures for assessing methylation sensitivity by luciferase reporter assay. This protocol is adaptable to other enhancers and tissues.\n\nFor complete details on the use and execution of this protocol, please refer to Taege et al.1",
  "abstract_shown": true,
  "affiliations": [
    "Institute for Human Genetics, Division Epigenetics & Metabolism, University of Luebeck, Ratzeburger Allee 160, 23562 Luebeck, Germany",
    "Research Unit NeuroBiology of Diabetes, Institute for Diabetes and Obesity, Helmholtz Centre Munich, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Natalie Taege"
    },
    {
      "affs": [
        1
      ],
      "name": "Sonja C. Schriever"
    },
    {
      "affs": [
        0
      ],
      "name": "Henriette Kirchner"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dvvpht8n7ds2g.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104922",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13733459/",
  "id": "dvvpht8n7ds2g",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104922",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1312|Epigenetics and DNA Methylation"
    ]
  ],
  "openalex": "W7224159087",
  "pdf_url": "https://www.cell.com/star-protocols/pdf/S2666-1667(26)00575-7.pdf",
  "pmcid": "PMC13733459",
  "pmid": "42961906",
  "published": "2026-10-29",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/dvvpht8n7ds2g.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:23:14Z",
      "native_id": "PMC13733459",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104922"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Epigenetics and DNA Methylation",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol to identify DNA methylation-sensitive enhancer sites",
  "title_text": "Protocol to identify DNA methylation-sensitive enhancer sites",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:23:14Z",
  "url": "https://research.draconitic.com/paper/dvvpht8n7ds2g",
  "venue": "STAR Protocols",
  "versions": []
}