{
  "abstract": "Drought stress severely limits rice growth, while roots play a key role in sensing water deficits and triggering adaptive responses. To investigate time-dependent molecular responses in rice (Oryza sativa L. cv. Koshihikari) under drought stress induced by polyethylene glycol (PEG), seedlings were exposed to 10 and 20% PEG for 4 and 8 days, followed by morphological, proteomic, immunoblot, polymerase chain reaction, and ATP content analyses. Primary root length and total root fresh weight were significantly decreased with 20% PEG for 8 days compared with untreated. Proteomic analysis revealed changes in the abundance of rice root proteins under 20% PEG treatment between 4 and 8 days, with differentially abundant proteins enriched in Gene Ontology categories related to nuclear components, protein phosphorylation, and ATP binding. Selected proteins related to protein protection, redox-associated processes, and cytoskeletal responses were examined using immunoblot and polymerase chain reaction analyses. Glutathione S-transferase decreased and heat shock protein 70 increased with PEG for 8 days. The expression of NADPH-dependent codeinone reductase was upregulated and heat shock protein 17.9 was downregulated by PEG for 8 days. ATP content per unit fresh weight decreased in roots after PEG treatment. These results suggest that prolonged PEG-induced osmotic stress inhibits rice root growth through alterations in cellular energy status, the abundance of proteins involved in protein protection and glutathione S-transferase-mediated detoxification, and the expression of genes associated with reactive carbonyl species metabolism.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Applied Sciences and Engineering, Fukui University of Technology, Fukui 910-8505, Japan",
    "Department of Management Nutrition, Aichi Gakusen University, Okazaki 444-8520, Japan",
    "Center for Medical Science, Fujita Health University, Toyoake 470-1192, Japan",
    "Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sheikh Shohag"
    },
    {
      "affs": [
        1
      ],
      "name": "Hisateru Yamaguchi",
      "orcid": "0000-0002-4507-9598"
    },
    {
      "affs": [
        2
      ],
      "name": "Keisuke Hitachi",
      "orcid": "0000-0002-7300-5238"
    },
    {
      "affs": [
        2
      ],
      "name": "Kunihiro Tsuchida",
      "orcid": "0000-0002-3983-5756"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Setsuko Komatsu",
      "orcid": "0000-0002-4514-357X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dxa51hzy6x0n7.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198895",
  "fulltext_url": "https://europepmc.org/articles/PMC13664928",
  "id": "dxa51hzy6x0n7",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/19/8895",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Stress Responses and Tolerance"
    ]
  ],
  "openalex": "W7220847991",
  "pdf_url": "https://europepmc.org/articles/PMC13664928?pdf=render",
  "pmcid": "PMC13664928",
  "pmid": "42884139",
  "published": "2026-10-07",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/dxa51hzy6x0n7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27198895",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/19/8895"
    },
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42884139",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42884139"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Stress Responses and Tolerance",
      "subfield": "plant-biology"
    }
  ],
  "title": "Proteomic and Molecular Biological Analyses of Response Mechanism in Rice (Oryza sativa L. cv. Koshihikari) Under Drought Stress",
  "title_text": "Proteomic and Molecular Biological Analyses of Response Mechanism in Rice (Oryza sativa L. cv. Koshihikari) Under Drought Stress",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/dxa51hzy6x0n7",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}