{
  "abstract": "TnpBs are compact RNA-guided nucleases ideally suited for in vivo delivery. By screening novel TnpBs identified from large metagenomic datasets, ISPmu1 was discovered and its protein and {omega}RNA were engineered to generate En-ISPmu1, which achieved editing efficiencies exceeding 65% across 12 human loci. Owing to its compact size, En-ISPmu1 was delivered together with a donor DNA in a single AAV vector, yielding up to 12.7% targeted integration in mouse liver. These findings establish En-ISPmu1 as a promising versatile platform for in vivo therapeutic genome editing applications.",
  "abstract_shown": true,
  "affiliations": [
    "University Trento"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "A. Conci"
    },
    {
      "name": "I. Bonuzzi"
    },
    {
      "name": "M. Ciciani"
    },
    {
      "name": "F. Esposito"
    },
    {
      "name": "P. Piccolo"
    },
    {
      "name": "F. Collaud"
    },
    {
      "name": "G. Ronzitti"
    },
    {
      "name": "A. Auricchio"
    },
    {
      "name": "N. Segata"
    },
    {
      "affs": [
        0
      ],
      "name": "A. Cereseto"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dxm1p95mkgxzh.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.26.761871",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761871v1.full",
  "id": "dxm1p95mkgxzh",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761871v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7224090628",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761871v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dxm1p95mkgxzh.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:59Z",
      "native_id": "10.64898/2026.10.26.761871",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.26.761871v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Single-vector AAV delivery of En-ISPmu1 and donor DNA for in vivo targeted genome integration",
  "title_text": "Single-vector AAV delivery of En-ISPmu1 and donor DNA for in vivo targeted genome integration",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:59Z",
  "url": "https://research.draconitic.com/paper/dxm1p95mkgxzh",
  "venue": "bioRxiv",
  "versions": []
}