{
  "abstract": "Background: Biofilm formation and quorum sensing (QS) make Pseudomonas aeruginosa and Staphylococcus aureus hard to treat, and many preclinical agents are reported to act against both.\n\nObjective: We mapped preclinical antibiofilm and anti-QS agents against these pathogens and graded how directly each study tested QS.\n\nMethods: PubMed and Scopus were searched without a date limit (3,349 records). Eligible in vitro and in vivo studies were published from 2022 onward, tested an exogenous agent and named either organism in the title. Full-text data were extracted, each study received a QS evidence tier, and risk of bias was appraised with an adapted checklist (in vitro) or SYRCLE's tool (vertebrate studies); two reviewers verified the extraction.\n\nResults: We included 252 studies, led by natural products (115). A QS assay in the target pathogen was present in 140 studies (56%), a surrogate reporter alone in 27 (11%), and 31 (12%) used a vertebrate infection model. In vitro risk of bias was low in 143, moderate in 93 and high in 16. No meta-analysis was done, and title-only organism matching may have missed studies.\n\nConclusion: Direct QS evidence is common for P. aeruginosa but thinner for S. aureus, and animal and cytotoxicity data are sparse.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Al-Salt, Jordan."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sham Ma’aitah"
    },
    {
      "affs": [
        0
      ],
      "name": "Batool AlAmairyin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dxxjth88nk6qe.bib",
  "cited_by": 0,
  "doi": "10.51152/jbarbiomed.v12i1.267",
  "fulltext_url": "https://jbarbiomed.com/home/article/view/267",
  "id": "dxxjth88nk6qe",
  "issns": [
    "2710-1479"
  ],
  "landing_url": "https://doi.org/10.51152/jbarbiomed.v12i1.267",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ],
    [
      "lcc",
      "R"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "Journal of Basic and Applied Research in Biomedicine",
  "ris": "https://research.draconitic.com/paper/dxxjth88nk6qe.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T14:21:05Z",
      "native_id": "eba64af4ab4e449881ef2c4c449bb70d",
      "source": "doaj",
      "url": "https://doi.org/10.51152/jbarbiomed.v12i1.267"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "lcc:QH301-705.5",
      "subfield": "general"
    },
    {
      "field": "medicine",
      "origin": "lcc:R",
      "subfield": "general"
    }
  ],
  "title": "Antibiofilm and Quorum-sensing Agents Against Pseudomonas aeruginosa and Staphylococcus aureus: a Systematic Review of Preclinical Studies, 2022-2026",
  "title_text": "Antibiofilm and Quorum-sensing Agents Against Pseudomonas aeruginosa and Staphylococcus aureus: a Systematic Review of Preclinical Studies, 2022-2026",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T14:20:31Z",
  "url": "https://research.draconitic.com/paper/dxxjth88nk6qe",
  "venue": "Journal of Basic and Applied Research in Biomedicine",
  "versions": []
}