{
  "abstract": "Cigarette smoke extract (CSE) exerts paradoxical concentration-dependent effects on vascular smooth muscle cells, though the underlying isoform-level mechanisms remain unexplored. We employed Oxford Nanopore long-read sequencing to analyze the full-length transcriptome of human aortic smooth muscle cells after CSE treatment. We confirmed that low-concentration CSE (0.2%) slightly promoted cell viability while higher concentrations (0.4%−0.8%) induced dose-dependent suppression. Sequencing of 0.6% CSE (midpoint cytotoxicity)-treated cells revealed 11,222 novel transcripts and extensive post-transcriptional reprogramming, including: 1,866 upregulated transcripts involving ferroptosis and cell division; 493 downregulated transcripts in oxidative phosphorylation and glutathione metabolism; 1,753 alternative polyadenylation events mediating 3’UTR remodeling; and 269 alternative splicing events affecting cell cycle regulators and transcription factors (CREM, FOSL1, HBP1). Our study suggests that high-concentration CSE cytotoxicity is executed through coordinated post-transcriptional dysregulation, providing the first isoform-level perspective on smoking-induced vascular pathology and revealing previously unrecognized regulatory mechanisms in cigarette smoke-mediated vascular injury.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Vascular Surgery, General Hospital of Ningxia Medical University, Yinchuan City, Ningxia, China",
    "Department of Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan City, Ningxia, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wei Gou"
    },
    {
      "affs": [
        1
      ],
      "name": "Lei Yang"
    },
    {
      "name": "Shan Chen"
    },
    {
      "name": "Yulin Miao"
    },
    {
      "name": "Weiwei Wang"
    },
    {
      "name": "Hai Li"
    },
    {
      "name": "Zhipeng Hu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dya4k5kq2r2tc.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0354604",
  "fulltext_url": null,
  "id": "dya4k5kq2r2tc",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0354604",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/Messenger RNA/Untranslated regions/3' UTR"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/RNA processing/Alternative splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/DNA-binding proteins/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Regulatory proteins/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Computational biology/Genome analysis/Transcriptome analysis"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Transcriptional control"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Polyadenylation"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/RNA processing/Alternative splicing"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Genomics/Genome analysis/Transcriptome analysis"
    ],
    [
      "plos",
      "/Biology and life sciences/Psychology/Behavior/Habits/Smoking habits"
    ],
    [
      "plos",
      "/Social sciences/Psychology/Behavior/Habits/Smoking habits"
    ]
  ],
  "openalex": "W7213957559",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0354604&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-21",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/dya4k5kq2r2tc.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:43Z",
      "native_id": "10.1371/journal.pone.0354604",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0354604"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A comprehensive full-length transcriptome landscape of cigarette smoke-exposed HASMCs reveals extensive remodeling of mRNA isoforms and regulatory networks",
  "title_text": "A comprehensive full-length transcriptome landscape of cigarette smoke-exposed HASMCs reveals extensive remodeling of mRNA isoforms and regulatory networks",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:43Z",
  "url": "https://research.draconitic.com/paper/dya4k5kq2r2tc",
  "venue": "PLOS One",
  "versions": []
}