{
  "abstract": "Analysis of mitochondrial morphology and ultrastructure from transmission electron microscopy images is essential for understanding cellular adaptations in both physiological and pathological conditions. However, these investigations remain time-consuming, poorly standardized, and therefore highly variable. We developed an open-access, standalone MATLAB-based application [Analysis of MITOchondria (AMITO)] that can be run without a license and integrates analyses within a single user-friendly interface. AMITO demonstrated excellent agreement with conventional manual image analysis software, high intra- and interexperimenter reproducibility, and substantial reduction in analysis time. Therefore, AMITO provides a reliable, efficient, and accessible tool to ensure robustness and reproducibility in mitochondrial assessment. AMITO is an open-access, standalone application for mitochondrial analysis from TEM images that combines automated and manual workflows within a single user-friendly interface. Validated against conventional FIJI analysis, AMITO demonstrated excellent reproducibility while substantially reducing analysis time and experimenter-dependent variability. By integrating morphology and ultrastructure quantification without requiring programming expertise, AMITO provides a reliable and accessible solution to improve robustness, efficiency, and reproducibility in mitochondrial research.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "Elyse Hucteau"
    },
    {
      "name": "Thomas J Hureau"
    },
    {
      "name": "Allan F Pagano"
    },
    {
      "name": "Joris Mallard"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dyybds9be116g.bib",
  "cited_by": 0,
  "doi": "10.1152/ajpcell.00300.2026",
  "fulltext_url": null,
  "id": "dyybds9be116g",
  "issns": [
    "0363-6143",
    "1522-1563"
  ],
  "landing_url": "https://univoak.hal.science/hal-05784199v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.ib"
    ],
    [
      "hal",
      "2.sdv.ib.ima"
    ],
    [
      "openalex",
      "1304|Cell Image Analysis Techniques"
    ]
  ],
  "openalex": "W7203724401",
  "pdf_url": "https://univoak.hal.science/hal-05784199/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-18",
  "publisher": "American Physiological Society",
  "ris": "https://research.draconitic.com/paper/dyybds9be116g.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "hal-05784199",
      "source": "hal",
      "url": "https://univoak.hal.science/hal-05784199v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.ib",
      "subfield": "systems-synthetic"
    },
    {
      "field": "medicine",
      "origin": "hal:2.sdv.ib.ima",
      "subfield": "imaging"
    }
  ],
  "title": "AMITO: an open-access application for assisted analysis of mitochondria in transmission electron microscopy images",
  "title_text": "AMITO: an open-access application for assisted analysis of mitochondria in transmission electron microscopy images",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:50Z",
  "url": "https://research.draconitic.com/paper/dyybds9be116g",
  "venue": "American Journal of Physiology - Cell Physiology",
  "versions": []
}