{
  "abstract": "Although Metschnikowia pulcherrima shows promise as a biocontrol agent, its upstream development remains limited. We maximized the viable cell count of M. pulcherrima strain APC 1.2 by employing a Central Composite Design (CCD) to optimize pH and temperature. Preliminary screening identified sucrose at 20 g/L and 1600 mg YAN/L yeast extract as optimal for viable cell count (CFU/mL), with further increases in YAN providing no additional benefit. The highest viable cell count was achieved at milder temperatures (18–22 °C), where the strain grew robustly across a wide pH range (3.59–6.41), while higher temperatures increased sensitivity to acidic pH and reduced viable cell count. The strain was confirmed to be Crabtree-negative, showing no ethanol production during aerobic growth on glucose. The proposed workflow enabled reproducible optimization of cultivation parameters, providing a scalable framework for industrial applications, and laying the groundwork for future studies on non-conventional yeasts in biocontrol and industrial biotechnology.",
  "abstract_shown": true,
  "affiliations": [
    "Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland; ETH Zürich, Institute of Food, Nutrition, and Health, Department of Health Sciences and Technology, Zurich, 8092, Switzerland; Corresponding authors at: Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland.",
    "ETH Zürich, Institute of Food, Nutrition, and Health, Department of Health Sciences and Technology, Zurich, 8092, Switzerland",
    "Agroscope, Department of Plant Protection, Nyon, 1260, Switzerland",
    "Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland; Corresponding authors at: Agroscope, Department of Food Microbial Systems, Posieux, 1725, Switzerland."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Bogdan T. Dinić"
    },
    {
      "affs": [
        1
      ],
      "name": "Nicholas A. Bokulich"
    },
    {
      "affs": [
        2
      ],
      "name": "Florian M. Freimoser"
    },
    {
      "affs": [
        3
      ],
      "name": "Ueli von Ah"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dzd3tr0cj0nhn.bib",
  "cited_by": 0,
  "doi": "10.1016/j.btre.2026.e00984",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2215017X26000391",
  "id": "dzd3tr0cj0nhn",
  "issns": [
    "2215-017X"
  ],
  "landing_url": "https://doi.org/10.1016/j.btre.2026.e00984",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "lcc",
      "TP248.13-248.65"
    ],
    [
      "openalex",
      "1106|Advanced Scientific Research Methods"
    ]
  ],
  "openalex": "W7221365927",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S2215017X26000391/pdf",
  "pmcid": null,
  "pmid": "42959835",
  "published": "2026-10-10",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/dzd3tr0cj0nhn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:44:38Z",
      "native_id": "f74432951431470ea303da234fa21111",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.btre.2026.e00984"
    },
    {
      "first_seen": "2026-11-01T00:20:52Z",
      "native_id": "MED:42959835",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42959835"
    },
    {
      "first_seen": "2026-11-02T12:22:29Z",
      "native_id": "PMC13718474",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.btre.2026.e00984"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "lcc:TP248.13-248.65",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Bioprocess optimization of",
  "title_text": "Bioprocess optimization of",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:29Z",
  "url": "https://research.draconitic.com/paper/dzd3tr0cj0nhn",
  "venue": "Biotechnology Reports",
  "versions": []
}