{
  "abstract": "Mansoa alliacea is traditionally used to treat inflammation, infections, and metabolic disorders. However, the phytochemical and biological potential of its flowers remains poorly characterized. This study optimized ultrasound-assisted extraction (UAE) of polyphenols from M. alliacea flowers and evaluated the in vitro antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities of the optimized extract. Four extraction parameters (ethanol concentration, extraction temperature, extraction time, and solid-to-solvent ratio) were initially screened by single-factor experiments. Based on these results, three key factors (temperature, time, and ethanol concentration) were selected and further optimized using a Box-Behnken design to maximize polyphenol yield. Total polyphenol content was quantified using the Folin-Ciocalteu method. UHPLC-MS profiling was conducted for tentative phytochemical characterization. The optimized extract was evaluated for in vitro antioxidant activity (DPPH, ABTS assays), anti-inflammatory activity (protein denaturation and RBC membrane stabilization), and digestive enzyme inhibitory activities (inhibition of α-amylase and α-glucosidase). Optimal extraction conditions (60% ethanol, 79 °C, 15 min, 1:10 w/v) yielded 275.37 ± 1.55 mg GAE/g extract. UHPLC-MS analysis revealed nine major peaks, among which two compounds were provisionally assigned as luteolin-7-O-glucoside and luteolin-7-O-glucuronide. Compared with the unoptimized extract, the optimized extract exhibited markedly stronger in vitro biological activities, with IC50 values of 11.88 ± 0.28 and 15.58 ± 0.33 μg/mL in DPPH and ABTS assays, respectively; 38.77 ± 0.93 μg/mL for inhibition of protein denaturation; 30.74 ± 0.18 μg/mL for RBC membrane stabilization; and 30.94 ± 0.54 and 13.32 ± 0.19 μg/mL for α-amylase and α-glucosidase inhibition, respectively. Optimized UAE significantly enhanced polyphenol enrichment in the extract and improved the in vitro antioxidant capacity, protective effects against protein denaturation and RBC hemolysis, and carbohydrate-hydrolyzing enzyme inhibitory activities of M. alliacea flower extract. These findings provide preliminary scientific support for the traditional use of M. alliacea and suggest that its polyphenol-rich extract may serve as a promising candidate for further investigation as a functional or phytopharmaceutical ingredient.",
  "abstract_shown": true,
  "affiliations": [
    "Faculty of Chemistry, University of Science, Ho Chi Minh City, 700000, Viet Nam.",
    "Vietnam National University, Ho Chi Minh City, 700000, Viet Nam.",
    "Faculty of Medicine, College of Health Sciences, Nam Can Tho University, 168 Nguyen Van Cu Extension Street, An Binh Ward, Can Tho, 94000, Viet Nam."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Quoc Trung Nguyen"
    },
    {
      "affs": [
        2
      ],
      "name": "Tran Chi Linh"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dzmt9zpa6m4pd.bib",
  "cited_by": 0,
  "doi": "10.1016/j.bbrep.2026.102851",
  "fulltext_url": "https://europepmc.org/articles/PMC13744347",
  "id": "dzmt9zpa6m4pd",
  "issns": [
    "2405-5808"
  ],
  "landing_url": "https://europepmc.org/article/MED/42999665",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2704|Phytochemicals and Antioxidant Activities"
    ]
  ],
  "openalex": "W7224989508",
  "pdf_url": "https://europepmc.org/articles/PMC13744347?pdf=render",
  "pmcid": "PMC13744347",
  "pmid": "42999665",
  "published": "2026-10-31",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/dzmt9zpa6m4pd.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:34Z",
      "native_id": "MED:42999665",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42999665"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2704|Phytochemicals and Antioxidant Activities",
      "subfield": "general"
    }
  ],
  "title": "Optimization of ultrasound-assisted extraction of polyphenols from Mansoa alliacea flowers and evaluation of their antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities.",
  "title_text": "Optimization of ultrasound-assisted extraction of polyphenols from Mansoa alliacea flowers and evaluation of their antioxidant, anti-inflammatory-related, and digestive enzyme inhibitory activities.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:34Z",
  "url": "https://research.draconitic.com/paper/dzmt9zpa6m4pd",
  "venue": "Biochemistry and biophysics reports",
  "versions": []
}