{
  "abstract": "The claustrum (CLA) receives dense serotonergic innervation from dorsal raphe nucleus (DRN) serotonin (5-HT) neurons, which are implicated in regulating sleep and stress homeostasis. However, the mechanisms by which 5-HT regulates the excitability of CLA neurons remain poorly defined. In the present study, we combined whole-cell patch-clamp recordings, 5-HT uncaging, and receptor pharmacology to examine the effects of 5-HT on the excitability of CLA neurons and their glutamatergic synapses. We found that 5-HT consistently induced membrane hyperpolarization or depolarization in regular-spiking type I and type II neurons, whereas it predominantly induced hyperpolarization in strongly adapting CLA neurons. The 5-HT-induced membrane hyperpolarization and depolarization were mediated by 5-HT1A receptors (5-HT1ARs) and 5-HT2A receptors (5-HT2ARs), respectively. Furthermore, we showed that activation of presynaptic 5-HT2ARs inhibits glutamatergic synaptic transmission in the CLA by reducing glutamate release. This effect was not mediated by the canonical phospholipase C (PLC) signaling cascade. Collectively, these findings reveal cell type-specific and multifaceted serotonergic modulation of the intrinsic excitability and synaptic transmission in the claustrum.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmacology and Toxicology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, United States",
    "University at Buffalo Neuroscience Program, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, United States",
    "Department of Neurobiology and Behavior, Cornell University, Ithaca, NY, United States"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Renee Rouleau"
    },
    {
      "affs": [
        0
      ],
      "name": "Saida Oubraim"
    },
    {
      "affs": [
        2
      ],
      "name": "Lizemarie Cirone"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Roh-Yu Shen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Samir Haj-Dahmane"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/dzsktt5ctw2sf.bib",
  "cited_by": 0,
  "doi": "10.3389/fncel.2026.1951375",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13710022/",
  "id": "dzsktt5ctw2sf",
  "issns": [
    "1662-5102"
  ],
  "landing_url": "https://doi.org/10.3389/fncel.2026.1951375",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2804|Neuroscience and Neuropharmacology Research"
    ]
  ],
  "openalex": "W7221724156",
  "pdf_url": "https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2026.1951375/pdf",
  "pmcid": "PMC13710022",
  "pmid": "42947812",
  "published": "2026-10-13",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/dzsktt5ctw2sf.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:04Z",
      "native_id": "PMC13710022",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fncel.2026.1951375"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2804|Neuroscience and Neuropharmacology Research",
      "subfield": "neuroscience"
    }
  ],
  "title": "Serotonergic regulation of neuronal excitability and glutamatergic synaptic transmission in rat claustrum",
  "title_text": "Serotonergic regulation of neuronal excitability and glutamatergic synaptic transmission in rat claustrum",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:04Z",
  "url": "https://research.draconitic.com/paper/dzsktt5ctw2sf",
  "venue": "Frontiers in Cellular Neuroscience",
  "versions": []
}