{
  "abstract": "Transgenic GAL4 lines are widely used in Drosophila to label and manipulate specific cell types, but the resulting reporter expression patterns do not always represent ongoing driver activity. We generated a collection of neuropeptide T2A-GAL4 knock-ins and characterized their expression by native-fluorescence imaging using UAS-CD8::GFP and a brp-miRFP680 knock-in, eliminating the need for immunostaining. During characterization, we observed both timing-dependent changes in adult GFP labeling and GAL4-associated cellular abnormalities. Using temperature-sensitive GAL80, we found that adult GFP labeling in certain neurons driven by CCHa2-T2A-GAL4 and Dsk-T2A-GAL4 depended on GAL4 activity before eclosion and was not reproduced by adult-restricted induction. Several GAL4 lines also showed dominant developmental phenotypes. Heterozygous Burs-T2A-GAL4 animals, for example, displayed wing expansion defects that were fully suppressed by GAL80. Severe wing defects were not accompanied by a reduction in the number of reporter-labeled neurons before eclosion, but the morphology of these neurons was affected. These observations illustrate how developmental driver history and GAL4-associated cellular effects can influence reporter patterns in the adult nervous system.",
  "abstract_shown": true,
  "affiliations": [
    "Tokyo University of Science"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "K. Jinnai"
    },
    {
      "name": "K. Ozawa"
    },
    {
      "name": "K. Yajima"
    },
    {
      "name": "H. Tanimoto"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Kondo"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e03tj9ddx8tjg.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.16.750837",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.16.750837v1.full",
  "id": "e03tj9ddx8tjg",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.16.750837v1",
  "language": null,
  "licence": "PDM-1.0",
  "licence_label": "Public domain",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/publicdomain/mark/1.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genetics"
    ],
    [
      "openalex",
      "2804|Neurobiology and Insect Physiology Research"
    ]
  ],
  "openalex": "W7214049860",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.16.750837v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-16",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/e03tj9ddx8tjg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:23:58Z",
      "native_id": "10.64898/2026.09.16.750837",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.16.750837v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:genetics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Neuropeptide T2A-GAL4 knock-ins illustrate practical considerations for interpreting GAL4 reporter patterns in Drosophila",
  "title_text": "Neuropeptide T2A-GAL4 knock-ins illustrate practical considerations for interpreting GAL4 reporter patterns in Drosophila",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:58Z",
  "url": "https://research.draconitic.com/paper/e03tj9ddx8tjg",
  "venue": "bioRxiv",
  "versions": []
}