{
  "abstract": "Peripheral blood-derived induced pluripotent stem cells (iPSCs) are attractive for clinical applications, yet multi-plasmid episomal protocols reduce delivery completeness and complicate good manufacturing practice (GMP) workflows. Here, we assembled OCT4, SOX2, MYC, BCL-XL, and KLF4 into a single OriP/EBNA1 episome and tuned KLF4 expression by placing it under the elongation factor 1 alpha (EF1α) promoter with 2 tandem miR-142-3p target sites in its 3'UTR. Across donors, the single-episome system produced robust day-14 colony yields normalized per 1 × 105 input cells. A green fluorescent protein (GFP) reporter demonstrated hematopoietic miR-142-dependent suppression, and the miR-gated construct exhibited significantly faster EBNA1 sequence loss by day 4. Resulting iPSC lines expressed TRA-1-60/TRA-1-81, SSEA4, NANOG, and OCT4, maintained normal karyotypes, and generated derivatives of all three germ layers. This miR-142-gated EF1α-KLF4 single-episome streamlines peripheral blood mononuclear cells (PBMCs) reprogramming and provides a GMP-amenable, non-integrating route to high-quality iPSCs.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.",
    "Tianjin Institutes of Health Science, Tianjin 301600, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiang Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Wei Wen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jian-Qiao Chang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Feng Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhu-Ying Gao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Shu-Man Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Guo-Hua Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Bin Liu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jue Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hang Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Chong-Yan Cheng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Li-Ming Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jian-Ping Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiao-Bing Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e1zwec0n5z3px.bib",
  "cited_by": 0,
  "doi": "10.1097/bs9.0000000000000277",
  "fulltext_url": "https://europepmc.org/articles/PMC13743012",
  "id": "e1zwec0n5z3px",
  "issns": [
    "2543-6368"
  ],
  "landing_url": "https://europepmc.org/article/MED/42997867",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Pluripotent Stem Cells Research"
    ]
  ],
  "openalex": "W7225430677",
  "pdf_url": "https://europepmc.org/articles/PMC13743012?pdf=render",
  "pmcid": "PMC13743012",
  "pmid": "42997867",
  "published": "2026-11-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/e1zwec0n5z3px.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:34Z",
      "native_id": "MED:42997867",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42997867"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Pluripotent Stem Cells Research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Single-episome delivery of BOSMK with miR-142-gated KLF4 streamlines PBMC reprogramming to iPSCs.",
  "title_text": "Single-episome delivery of BOSMK with miR-142-gated KLF4 streamlines PBMC reprogramming to iPSCs.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:34Z",
  "url": "https://research.draconitic.com/paper/e1zwec0n5z3px",
  "venue": "Blood science (Baltimore, Md.)",
  "versions": []
}