{
  "abstract": "Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive and lethal malignancies, with limited therapeutic options. Despite advances in chemotherapy, such as FOLFIRINOX and Abraxane® (nab-paclitaxel) combined with gemcitabine, these treatments show limited efficacy due to systemic toxicity, poor tumor specificity, and drug resistance. Nanomedicine offers a promising strategy to improve drug delivery, reduce off-target effects, and enhance therapeutic efficacy. In this study, we developed and physicochemically characterized paclitaxel (PTX)-loaded nanostructured lipid carriers (NLC) surface-functionalized with the nucleolin-targeting pseudopeptide N6L, prepared by the solvent displacement method and coated via electrostatic adsorption. Diluted PTX-loaded NLC displayed a mean hydrodynamic diameter of 165 ± 5 nm, a polydispersity index of 0.14 ± 0.09, and a zeta potential of -28 ± 1 mV. Following N6L adsorption, the zeta potential shifted to + 40 mV, confirming successful surface functionalization, while drug loading corresponded to 1.48 µg PTX per mg of lyophilized formulation (0.148% w/w). Freeze-drying with 25% (w/v) sorbitol as a cryoprotectant preserved particle size and colloidal integrity upon redispersion, supporting suitability for long-term storage. Cytotoxicity assays on PANC-1 pancreatic cancer cells using the MTT assay revealed that N6L-functionalized PTX-loaded NLC reduced cell viability to 23% at the highest concentration tested, corresponding to an approximately 20% increase in cytotoxicity compared with blank NLC. These preliminary results demonstrate the feasibility of engineering N6L-functionalized PTX-loaded NLC with suitable physicochemical properties, colloidal stability, and antitumor activity, supporting their potential as a nanocarrier platform for pancreatic cancer therapy.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Laura Larue"
    },
    {
      "name": "Blandine Brissault"
    },
    {
      "name": "Cléa Chesneau"
    },
    {
      "name": "Claire Houppe"
    },
    {
      "name": "Laurent Michely"
    },
    {
      "name": "Eric Leroy"
    },
    {
      "name": "Ilaria Cascone"
    },
    {
      "name": "Sabrina Belbekhouche"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e2dwnqd2ejpqa.bib",
  "cited_by": 0,
  "doi": "10.1016/j.biopha.2026.119797",
  "fulltext_url": null,
  "id": "e2dwnqd2ejpqa",
  "issns": [
    "0753-3322",
    "1950-6007"
  ],
  "landing_url": "https://hal.science/hal-05837230v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.chim"
    ],
    [
      "openalex",
      "2502|Nanoparticle-Based Drug Delivery"
    ]
  ],
  "openalex": "W7172454375",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/e2dwnqd2ejpqa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:01Z",
      "native_id": "hal-05837230",
      "source": "hal",
      "url": "https://hal.science/hal-05837230v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2502|Nanoparticle-Based Drug Delivery",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Paclitaxel-loaded nanostructured lipid carriers surface-functionalized with N6L for pancreatic cancer",
  "title_text": "Paclitaxel-loaded nanostructured lipid carriers surface-functionalized with N6L for pancreatic cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:01Z",
  "url": "https://research.draconitic.com/paper/e2dwnqd2ejpqa",
  "venue": "Biomedicine and Pharmacotherapy",
  "versions": []
}