{
  "abstract": "Ankyrin repeat domain-containing protein 55 (ANKRD55) is genetically associated with multiple sclerosis, but its cellular function remains incompletely defined. To investigate its potential role in neuroinflammatory pathways, we mapped ANKRD55-interacting proteins in neuroblastoma, astrocytic, microglial, and monocytic cell lines by affinity purification–mass spectrometry. Across all four cell lines, 14-3-3 isoforms were the most reproducible partners, whereas the IMhu-M microglial interactome uniquely contained eight intraflagellar transport complex B (IFT-B) components. In visible immunoprecipitation assays, IFT46–IFT56 was the smallest tested IFT-B subassembly sufficient to support detectable association with ANKRD55. Confocal microscopy localized endogenous ANKRD55 to pericentriolar material and basal bodies, but not to primary cilia, and identified partial association with P-body markers. These data distinguish a conserved 14-3-3 interaction core from cell-restricted centrosomal/IFT-B and RNP-associated ANKRD55 pools.",
  "abstract_shown": true,
  "affiliations": [
    "Inflammation & Biomarkers Group, Biobizkaia Health Research Institute, 48903 Barakaldo, Spain",
    "Red de Enfermedades Inflamatorias (REI), Redes de Investigación Cooperativa Orientada a Resultados en Salud (RICORS), Carlos IIII Health Research Institute, 28029 Madrid, Spain",
    "Microscopy Facility, Biobizkaia Health Research Institute, 48903 Barakaldo, Spain",
    "Proteomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), CIBERehd, Bizkaia Science and Technology Park, 48160 Derio, Spain",
    "Department of Immunology, Genetics and Pathology, Cancer Precision Medicine Research Unit, Rudbeck Laboratory, Uppsala University, 75185 Uppsala, Sweden",
    "Department of Neuroscience, Faculty of Medicine and Nursing, University of the Basque Country (EHU), 48940 Leioa, Spain",
    "Department of Physiology, Faculty of Medicine and Nursery, University of the Basque Country (EHU), 48940 Leioa, Spain",
    "Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan",
    "Biochemistry and Molecular Biology Department, Science and Technology School, University of the Basque Country (EHU), 48940 Leioa, Spain",
    "Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jorge Mena"
    },
    {
      "affs": [
        0
      ],
      "name": "Raquel Tulloch-Navarro"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ainhoa Fiat-Arriola",
      "orcid": "0009-0001-4036-0479"
    },
    {
      "affs": [
        2
      ],
      "name": "Javier Díez-García",
      "orcid": "0000-0003-2801-0721"
    },
    {
      "affs": [
        3
      ],
      "name": "Mikel Azkargorta"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ane Aldekoa-Etxabe"
    },
    {
      "affs": [
        0
      ],
      "name": "Nerea Ugidos-Damboriena"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Isabel Artiga-Folch"
    },
    {
      "affs": [
        4
      ],
      "name": "Cecilia Lindskog"
    },
    {
      "affs": [
        5
      ],
      "name": "Olatz Pampliega"
    },
    {
      "affs": [
        0,
        1,
        6
      ],
      "name": "Iraide Alloza-Moral"
    },
    {
      "affs": [
        7
      ],
      "name": "Yohei Katoh"
    },
    {
      "affs": [
        7
      ],
      "name": "Kazuhisha Nakayama"
    },
    {
      "affs": [
        3
      ],
      "name": "Felix Elortza",
      "orcid": "0000-0001-8839-5438"
    },
    {
      "affs": [
        0,
        1,
        8,
        9
      ],
      "name": "Koen Vandenbroeck van Caeckenbergh",
      "orcid": "0000-0002-6967-6485"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e34c2vsbefgbn.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16111620",
  "fulltext_url": null,
  "id": "e34c2vsbefgbn",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/11/1620",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/e34c2vsbefgbn.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T00:21:28Z",
      "native_id": "10.3390/biom16111620",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/11/1620"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-273X",
      "subfield": "general"
    }
  ],
  "title": "Comparative Interactomics Identifies Conserved 14-3-3 Interactions and an Association of ANKRD55 with IFT-B in a Microglial Cell Model",
  "title_text": "Comparative Interactomics Identifies Conserved 14-3-3 Interactions and an Association of ANKRD55 with IFT-B in a Microglial Cell Model",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T00:21:28Z",
  "url": "https://research.draconitic.com/paper/e34c2vsbefgbn",
  "venue": "Biomolecules",
  "versions": []
}