{
  "abstract": "Olive leaves (OL; Olea europaea L.) are an abundant by-product of olive production with functional and nutraceutical potential. This study evaluated the effects of post-harvest treatments using fresh, air-dried, and lyophilized OL of cv. ‘Frantoio’, which were infused in water at 85 °C for 10 min. The antioxidant capacity (AC; DPPH method), total phenolic content (TPC; Folin–Ciocalteu assay), and α-amylase inhibition activity of the herbal infusions were assessed. Macro- and trace elements were determined directly in air-dried and lyophilized OL by inductively coupled plasma optical emission spectrometry (ICP-OES). Potential non-carcinogenic health risks were assessed using estimated daily intake (EDI), target hazard quotient (THQ), and total THQ (TTHQ). Lyophilized OL herbal infusions exhibited the highest AC (15.32 ± 0.85%), TPC (65.61 ± 1.15 mg GAE/g DW), and α-amylase inhibition activity (23.34%). The mineral analysis identified Ca and K as the predominant macroelements, whereas Cd and Pb were below the analytical limit of detection. All individual THQ and cumulative TTHQ values remained below 1, indicating no appreciable non-carcinogenic health risk under the modeled exposure conditions. These results identify lyophilization as a favorable processing option for preserving the functional potential of OL and support their potential use as raw material for functional infusions and other food applications.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia",
    "AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia",
    "Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia",
    "M. M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, Sadovo-Botanichna 1, 010 14 Kyiv, Ukraine",
    "Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Drazovska 4, 949 74 Nitra, Slovakia",
    "Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlin, Vavreckova 5669, 760 01 Zlin, Czech Republic",
    "Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Liliana Hnatova"
    },
    {
      "affs": [
        0
      ],
      "name": "Zuzana Knazicka",
      "orcid": "0009-0001-1872-2920"
    },
    {
      "affs": [
        0
      ],
      "name": "Eva Kovacikova",
      "orcid": "0000-0003-3019-7812"
    },
    {
      "affs": [
        0
      ],
      "name": "Branislav Galik",
      "orcid": "0000-0001-6852-7659"
    },
    {
      "affs": [
        0
      ],
      "name": "Katarina Fatrcova Sramkova"
    },
    {
      "affs": [
        0
      ],
      "name": "Dominika Lenicka"
    },
    {
      "affs": [
        1
      ],
      "name": "Ivona Janco",
      "orcid": "0000-0002-9361-716X"
    },
    {
      "affs": [
        2
      ],
      "name": "Marian Tomka",
      "orcid": "0000-0003-2874-5701"
    },
    {
      "affs": [
        3
      ],
      "name": "Olga Grygorieva",
      "orcid": "0000-0003-1161-0018"
    },
    {
      "affs": [
        4
      ],
      "name": "Tunde Jurikova",
      "orcid": "0000-0002-8286-8262"
    },
    {
      "affs": [
        5
      ],
      "name": "Jiri Mlcek",
      "orcid": "0000-0002-5753-8560"
    },
    {
      "affs": [
        6
      ],
      "name": "Marcela Capcarova"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e3sk80v1eeeet.bib",
  "cited_by": 0,
  "doi": "10.3390/molecules31203735",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707913/?tool=EBI",
  "id": "e3sk80v1eeeet",
  "issns": [
    "1420-3049"
  ],
  "landing_url": "https://www.mdpi.com/1420-3049/31/20/3735",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1605|Edible Oils Quality and Analysis"
    ]
  ],
  "openalex": "W7222970266",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707913/pdf/?tool=EBI",
  "pmcid": "PMC13707913",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/e3sk80v1eeeet.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:42Z",
      "native_id": "10.3390/molecules31203735",
      "source": "crossref",
      "url": "https://www.mdpi.com/1420-3049/31/20/3735"
    },
    {
      "first_seen": "2026-10-30T12:21:44Z",
      "native_id": "PMC:PMC13707913",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13707913"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1605|Edible Oils Quality and Analysis",
      "subfield": "organic"
    }
  ],
  "title": "Olive Leaves for Functional Herbal Infusions: Effects of Post-Harvest Processing on Bioactive Properties, α-Amylase Inhibition Activity and Consumer Safety",
  "title_text": "Olive Leaves for Functional Herbal Infusions: Effects of Post-Harvest Processing on Bioactive Properties, α-Amylase Inhibition Activity and Consumer Safety",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:44Z",
  "url": "https://research.draconitic.com/paper/e3sk80v1eeeet",
  "venue": "Molecules",
  "versions": []
}